Leptin regulates olfactory-mediated behavior in ob/ob mice
Leptin regulates olfactory-mediated behavior in ob/ob mice
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DOI：
10.1016/j.physbeh.2005.11.016
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发表时间：
2006-05-30
影响因子：
2.9
通讯作者：
Getchell, Marilyn L.
中科院分区：
文献类型：
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作者：
Getchell, Thomas V.;Kwong, Kevin;Getchell, Marilyn L.
我们研究了嗅觉介导的瘦素（ob/ob）和瘦素受体（db/dh）突变小鼠与年龄和性别匹配的野生型（wt）小鼠的摄食前行为。嗅觉介导的行为进行了测试，使用一个埋藏的食物范例5次/天，2小时的间隔。6天。ob/ob和db/db小鼠的平均食物发现时间比野生型小鼠短约10倍。为了测试瘦素替代物在ob/ob小鼠中的作用，瘦素（1或5 μ g/g体重，在无菌盐水中）或载体。在测试前每天腹膜内注射一次。注射载体或1 μ g/g瘦素的ob/ob小鼠的平均食物发现时间相似，比野生型小鼠快2-3倍。注射5 μ g/g瘦素的ob/ob小鼠的平均食物发现时间是注射载体的ob/ob小鼠的三倍，与野生型小鼠的数量级相同，表明瘦素的功能性替代。三因素重复测量方差分析显示6个队列之间存在显著差异（P=0.0001），食物发现时间（P
We have investigated olfactory-mediated pre-ingestive behavior in leptin (ob/ob) and leptin receptor (db/dh) mutant mice compared to age- and gender-matched wild-type (wt) mice. Olfactory-mediated behavior was tested using a buried food paradigm 5 times/day at 2-h intervals. for 6 days. Mean food-finding times of ob/ob and db/db mice were approximately 10 times shorter than those of wt mice. To test the effect of leptin replacement in ob/ob mice, leptin (1 or 5 mu g/g body weight in sterile saline) or carrier. was injected i.p. once daily prior to testing. Mean food finding times in ob/ob mice injected with carrier or with 1 mu g/g leptin were similar and were 2-3 times faster than in wt mice. Mean food finding times in ob/ob mice injected with 5 mu g/g leptin tripled compared to carrier-injected ob/ob mice and were of the same order of magnitude as those of wt mice, suggesting functional leptin replacement. A 3-factor repeated measures ANOVA demonstrated significant differences between the 6 cohorts (P=0.0001), food finding times (P