TSH RECEPTOR GENE-EXPRESSION IN RETROOCULAR FIBROBLASTS
TSH RECEPTOR GENE-EXPRESSION IN RETROOCULAR FIBROBLASTS
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DOI：
10.1007/bf03347732
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发表时间：
1994-06-01
影响因子：
5.4
通讯作者：
BURMAN, KD
中科院分区：
文献类型：
--
作者：
MENGISTU, M;LUKES, YG;BURMAN, KD
从眼后区的成纤维细胞、从眼眶外直肌获得的肌内膜成纤维细胞和从腹部皮肤成纤维细胞中分离RNA。将RNA逆转录成cDNA，然后将其用作PCR的模板，引物包含人TSH受体（hTSH-R）的细胞外结构域的一部分（核苷酸989-1235）。用溴化乙锭染色法从成纤维细胞RNA中检测到247 BP的特异性产物，并与标记的hTSH-R cDNA杂交证实。该产物与已知的促甲状腺激素受体cDNA具有同源性。这些研究表明，人成纤维细胞可以表达hTSH-R，并且它们表明抗hTSH-R和这些成纤维细胞TSH受体之间的交叉反应性免疫应答可能在Graves眼病的发生中起作用。
RNA was isolated from fibroblasts from the retroocular area, from endomysial fibroblasts obtained from orbital lateral rectus muscle, and from abdominal skin fibroblasts. The RNA was reverse transcribed into cDNA which was then used as a template for PCR with primers encompassing a portion (nucleotides 989-1235) of the extra-cellular domain of the human TSH receptor (hTSH-R). A definite 247 BP product was detected from fibroblast RNA by ethidium bromide staining, and was confirmed by hybridization with labelled hTSH-R cDNA. The product had homology with the known TSH-R cDNA. These studies indicate that human fibroblasts can express hTSH-R, and they suggest that a cross reactive immunologic response between anti-hTSH-R and these fibroblast TSH receptors may play a role in the genesis of Graves' ophthalmopathy.