Effects of abscisic acid, salicylic acid, ethylene and hydrogen peroxide in thermotolerance and recovery for creeping bentgrass
Effects of abscisic acid, salicylic acid, ethylene and hydrogen peroxide in thermotolerance and recovery for creeping bentgrass
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DOI：
10.1007/s10725-005-1536-z
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发表时间：
2005-09-01
影响因子：
4.2
通讯作者：
Huang, BR
中科院分区：
文献类型：
--
作者：
Larkindale, J;Huang, BR
脱落酸（ABA）、水杨酸（SA）、乙烯和过氧化氢（H2O2）可能参与调控植物对热胁迫的反应。本研究的目的是确定这些信号分子是否参与匍匐弯草（Agrostis stolonifera）在高温下的存活。我们研究了ABA、SA、H2O2和ACC（乙烯前体）处理对匍匐弯草在高温胁迫（35℃持续1个月）下发生的生理损伤的影响。我们还比较了化学施用和中等热胁迫（30℃24 h）诱导的耐热性的影响。与对照植株相比，所有预处理（热处理或化学处理）都增加了对长时间热胁迫（1个月）的耐受性。与对照植物相比，所有处理的样品都显示出更多的绿叶，减少膜渗漏和减少氧化损伤。然后，我们测量了这些化学成分在热应激（35℃）和应激处理后恢复（冷却回20℃）期间内源性浓度的变化。热处理开始5 min后，氧化爆发，H2O2的增加主要发生在叶片和根组织细胞的外质体中。热应激开始后仅1小时检测到游离SA，随后浓度保持在较低水平。在热胁迫下，ABA和乙烯浓度均未升高，但在随后的冷却过程中，ABA和乙烯浓度均有所升高。这些结果表明，感兴趣的信号成分与匍匐弯草的耐热性有关，但不同的化学物质可能参与不同的信号通路。氧化破裂和SA可能是真正的热应激信号，但ABA和乙烯似乎参与了该物种从热应激中恢复的信号通路。
Abscisic acid (ABA), salicylic acid (SA), ethylene, and hydrogen peroxide (H2O2) may be involved in the regulation of plant responses to heat stress. The objective of this study was to determine whether these signaling molecules are involved in survival at high temperatures in creeping bentgrass (Agrostis stolonifera). We investigated the effects of treatment with ABA, SA, H2O2, and ACC (an ethylene precursor) on physiological damage occurring in creeping bentgrass during heat stress (35 degrees C for 1 month). We also compared the effects of chemical application and the induction of thermotolerance using moderate heat stress (30 degrees C for 24 h). All of the pre-treatments (heat or chemical) resulted in increased tolerance to prolonged heat stress (1 month) compared to control plants. All treated samples showed more green leaves, decreased membrane leakage and reduced oxidative damage compared to control plants. We then measured changes in the endogenous concentration of these chemical components during heat stress (35 degrees C) and during recovery after a stress treatment (cooling back to 20 degrees C). An oxidative burst was detected 5 min after the initiation of heat treatment, with the increase in H2O2 being detected primarily in the apoplast of the cells in both leaf and root tissues. Free SA was detected only an hour after the initiation of heat stress, and concentration remained low subsequently. Neither ABA nor ethylene concentrations rose during heat stress, but the concentration of both increased during subsequent cooling. These results suggest that the signaling components of interest are involved in thermotolerance in creeping bentgrass, but that the different chemicals are likely to be involved in separate signaling pathways. An oxidative burst and SA may be bona fide heat stress signals, but ABA and ethylene appear to be involved in signaling pathways in response to recovery from heat stress in this species.