Effects of Phosphoryl Choline Grafted Water Soluble Carbon Nanotubes Examined by Different Cytotoxicity Methods in 16-HEB Cells
Effects of Phosphoryl Choline Grafted Water Soluble Carbon Nanotubes Examined by Different Cytotoxicity Methods in 16-HEB Cells
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DOI：
10.4028/www.scientific.net/amr.486.84
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发表时间：
2012-03
期刊：
影响因子：
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通讯作者：
Yan Zhang;B. Ye;X. Wang;Y. Fu;Tao Zhang;X. Li;L. Yin;Yuepu Pu;Geyu Liang
中科院分区：
文献类型：
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作者：
Yan Zhang;B. Ye;X. Wang;Y. Fu;Tao Zhang;X. Li;L. Yin;Yuepu Pu;Geyu Liang
水溶性碳纳米管的广泛应用使得了解其对健康的潜在毒性作用变得非常重要。本研究通过不同的体外细胞毒性方法研究了磷酰胆碱接枝水溶性多壁碳纳米管（MWCNTs-PC）对人支气管上皮细胞（16-HBE）的影响。将不同浓度的MWCNTs-PC与16-HBE细胞孵育，分别采用甲基噻唑四氮唑（MTT）实验、流式细胞术、单细胞凝胶电泳（SCGE）实验和微核实验检测其对细胞增殖、细胞凋亡、细胞周期和DNA损伤的影响。与对照组相比，细胞增殖、细胞凋亡、细胞周期和DNA损伤的变化均无显著差异。在实验浓度范围内，本研究未观察到MWCNTs-PC对16-HBE细胞的明显细胞毒性和DNA损伤。
The widespread explored application of water soluble carbon nanotubes makes it important to understand their potential toxic effects on health. This study investigates the effects of phosphoryl choline grafted water soluble multi-walled carbon nanotubes (MWCNTs-PC) on human bronchial epithelial (16-HBE) cells by different cytotoxicity methods in vitro. Various concentrations of MWCNTs-PC were incubated with 16-HBE cells, the effects of cell proliferation, cell apoptosis, cell cycle and DNA damage were detected by methyl thiazolyl tetrazolium (MTT) assay, flow cytometry, single cell gel electrophoresis assay (SCGE) and micronuclear assay, respectively. Compared with the control group, there were no significant differences in the changes of cell proliferation, cell apoptosis, cell cycle and DNA damage. Within the experimental concentrations of MWCNTs-PC, no obviously cytotoxicity and DNA damage was observed on 16-HBE cells in this study.