Antibacterial effects of nanopillar surfaces are mediated by cell impedance, penetration and induction of oxidative stress
Antibacterial effects of nanopillar surfaces are mediated by cell impedance, penetration and induction of oxidative stress
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DOI：
10.1038/s41467-020-15471-x
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发表时间：
2020-04-02
影响因子：
16.6
通讯作者：
Su, B.
中科院分区：
文献类型：
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作者：
Jenkins, J.;Mantell, J.;Su, B.
一些昆虫，如蜻蜓，已经进化出翅膀上的纳米突起，可以在接触细菌时破裂。这启发了用合成材料制成的仿昆虫翅膀纳米柱设计抗菌植入物表面的灵感。在这里，我们描述了模拟钛纳米柱对细菌的生理和形态影响。纳米柱诱导革兰氏阳性和革兰氏阴性细菌细胞膜的变形和渗透，但不会使细菌破裂或溶解。它们还可以抑制细菌细胞分裂，触发活性氧的产生，增加氧化应激蛋白的丰度。我们的研究结果表明，纳米柱的抗菌活性可能是由氧化应激介导的，而不一定需要细菌裂解。类似于某些昆虫翅膀表面的合成纳米柱具有抗菌活性。在这里，Jenkins等人表明这些活动并不一定需要细菌裂解，可能是由细菌包膜变形引起的氧化应激介导的。
Some insects, such as dragonflies, have evolved nanoprotrusions on their wings that rupture bacteria on contact. This has inspired the design of antibacterial implant surfaces with insect-wing mimetic nanopillars made of synthetic materials. Here, we characterise the physiological and morphological effects of mimetic titanium nanopillars on bacteria. The nanopillars induce deformation and penetration of the Gram-positive and Gram-negative bacterial cell envelope, but do not rupture or lyse bacteria. They can also inhibit bacterial cell division, and trigger production of reactive oxygen species and increased abundance of oxidative stress proteins. Our results indicate that nanopillars' antibacterial activities may be mediated by oxidative stress, and do not necessarily require bacterial lysis. Synthetic nanopillars resembling the wing surface of certain insects display antibacterial activities. Here, Jenkins et al. show that these activities do not necessarily require bacterial lysis, and may be mediated by oxidative stress induced by deformation of the bacterial cell envelope.