Annotated draft genome sequences of three species of Cryptosporidium: Cryptosporidium meleagridis isolate UKMEL1, C. baileyi isolate TAMU-09Q1 and C. hominis isolates TU502 2012 and UKH1
Annotated draft genome sequences of three species of Cryptosporidium: Cryptosporidium meleagridis isolate UKMEL1, C. baileyi isolate TAMU-09Q1 and C. hominis isolates TU502 2012 and UKH1
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DOI：
10.1093/femspd/ftw080
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发表时间：
2016-10-01
影响因子：
3.3
通讯作者：
Silva, Joana C.
中科院分区：
文献类型：
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作者：
Ifeonu, Olukemi O.;Chibucos, Marcus C.;Silva, Joana C.
人类隐孢子虫病主要由人隐孢子虫、隐孢子虫C. parvum和C. meleagridis.为了加速隐孢子虫属寄生虫的研究，我们生成了人类隐孢子虫基因组序列的注释草图。人型分离株TU502_2012和UKH 1、C.也是从人类患者分离的C. agridis UKMEL 1和禽寄生虫C. baileyi TAMU-09Q1.基因组序列的注释部分依赖于从两种C. hominis和C.贝利伊。C.人的基因组比以前的基因组更完整，片段更少，这使得该物种的基因组得到了很大的改进，相对于2004年C. hominis注释。我们的研究结果表明，C。hominis和C.在基因密度和平均基因长度上，小孢子虫的基因密度和平均基因长度非常相似。这些数据对隐孢子虫研究界来说是一个有价值的资源。人型分离株TU502_2012和UKH 1;隐孢子虫（Cryptosporidiumbaileyi）、隐孢子虫（C.人型分离株TU502_2012和UKH 1;将加速隐孢子虫寄生虫的研究。
Human cryptosporidiosis is caused primarily by Cryptosporidium hominis, C. parvum and C. meleagridis. To accelerate research on parasites in the genus Cryptosporidium, we generated annotated, draft genome sequences of human C. hominis isolates TU502_2012 and UKH1, C. meleagridis UKMEL1, also isolated from a human patient, and the avian parasite C. baileyi TAMU-09Q1. The annotation of the genome sequences relied in part on RNAseq data generated from the oocyst stage of both C. hominis and C. baileyi. The genome assembly of C. hominis is significantly more complete and less fragmented than that available previously, which enabled the generation of a much-improved gene set for this species, with an increase in average gene length of 500 bp relative to the protein-encoding genes in the 2004 C. hominis annotation. Our results reveal that the genomes of C. hominis and C. parvum are very similar in both gene density and average gene length. These data should prove a valuable resource for the Cryptosporidium research community.The release of the draft genome sequence, and corresponding annotation, of Cryptosporidium baileyi, C. hominis isolates TU502_2012 and UKH1, and C. meleagridis, will accelerate research on Cryptosporidium parasites.The release of the draft genome sequence, and corresponding annotation, of Cryptosporidium baileyi, C. hominis isolates TU502_2012 and UKH1, and C. meleagridis, will accelerate research on Cryptosporidium parasites.