Direct tissue analysis of phospholipids in rat brain using MALDI-TOFMS and MALDI-ion mobility-TOFMS
Direct tissue analysis of phospholipids in rat brain using MALDI-TOFMS and MALDI-ion mobility-TOFMS
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DOI：
10.1016/j.jasms.2004.10.002
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发表时间：
2005-02-01
影响因子：
3.2
通讯作者：
Woods, AS
中科院分区：
文献类型：
--
作者：
Jackson, SN;Wang, HYJ;Woods, AS
除了水，脂质是大脑中发现的最常见的生物分子(12%)。简单阅读一下大脑的生理学、解剖学和病理生理学，就能说明脂类的重要性。质谱学的最新进展使直接探测组织成为可能。然而，大多数研究都集中在蛋白质上。本文采用基质辅助激光解吸电离飞行时间质谱仪(MALDI-TOFMS)和MALDI离子迁移率(IM)-TCFMS直接分析大鼠脑组织中的磷脂。记录了对应于磷脂酰胆碱、磷脂酰乙醇胺和鞘磷脂的分子离子(MH+)。在学习药理学时，我们了解到许多治疗化合物储存在人体的脂肪组织中。因此，用MALDI-TOFMS和MALDI-IM-TOFMS对直接加入氯异丹明的大鼠脑组织进行分析。用这两种技术检测到了组织磷脂与氯异丹明之间的非共价络合物。此外，磷脂和氯异丹明之间的非共价络合物的MALDI-IM-TOFMS显示了等量类脂和多肽之间的流动性。(C)2004年美国质谱学学会。
After water, lipids are the most common biomolecules found in the brain (12%). A brief perusal of the physiology, anatomy, and pathophysiology of the brain illustrates the importance of lipids. Recent advances in mass spectrometry have allowed the direct probing of tissues. However, most studies have focused on proteins. In the present work, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) and MALDI-ion mobility (IM)-TCFMS were employed for direct analysis of phospholipids in rat brain tissue. Molecular ions (MH+) corresponding to phosphatidylcholines, phosphatidylethanolamines, and sphingomyelin, were recorded. When studying pharmacology, we learn that many therapeutic compounds are stored in the body's adipose tissue. MALDI-TOFMS and MALDI-IM-TOFMS were thus used to analyze rat brain tissue with chlorisondamine added directly onto the tissue slice. With both techniques, noncovalent complexes between the tissue phospholipids and chlorisondamine were detected. In addition, MALDI-IM-TOFMS of noncovalent complexes between phospholipids and chlorisondamine displayed a mobility between that of an isobaric lipid and peptide. (C) 2004 American Society for Mass Spectrometry.