Molecular characterization, expression and chromosomal localization of porcine PNPLA3 and PNPLA4
Molecular characterization, expression and chromosomal localization of porcine PNPLA3 and PNPLA4
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猪 PNPLA3 和 PNPLA4 的分子特征、表达和染色体定位
DOI：
10.1007/s10529-011-0591-y
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发表时间：
2011
影响因子：
2.7
通讯作者：
Zaiqing Yang
中科院分区：
文献类型：
--
作者：
Zhilong Chen;Xin Gao;T. Lei;Xiaodong Chen;Lei Zhou;An Yu;Peng Lei;Rongrong Zhang;H. Long;Zaiqing Yang
Patatin-like phospholipases （PNPLAs）是一个蛋白质家族，其成员包含一个保守的具有脂质酰基水解酶活性的Patatin-like结构域。报道了猪PNPLA3和PNPLA4全长cdna的克隆和鉴定，并首次鉴定了猪PNPLA4的另一种剪接变体，该变体跳过了正常转录本的外显子5。随后，通过组织表达定量PCR分析发现，猪PNPLA3主要在脂肪和肝脏中表达，而猪PNPLA4在肝脏中表达丰富。猪的PNPLA3和PNPLA4分别位于5p11-p15和Xp24染色体上。
Patatin-like phospholipases (PNPLAs) comprise a protein family whose members contain a conserved patatin-like domain with lipid acyl hydrolase activity. The cloning and characterization of full-length cDNAs of PNPLA3 and PNPLA4 in pigs is reported and, for the first time, an alternative splicing variant of porcine PNPLA4, which skips exon 5 of the normal transcript, was identified. Subsequently, tissue expression analysis by quantitative PCR indicated that porcine PNPLA3 was mainly expressed in both adipose and liver whereas porcine PNPLA4 was abundant in liver. Porcine PNPLA3 and PNPLA4 were assigned respectively to chromosome 5p11-p15 and Xp24.
影响因子：
7.7
作者：
Kershaw, EE;Hamm, JK;Flier, JS
通讯作者：
Flier, JS
影响因子：
9.8
作者：
Ghosh, S;Watanabe, RM;Boehnke, M
通讯作者：
Boehnke, M