Chromosome Changes Revealed by the Q-Band Staining Method during Cell Senescence of WI-38
Chromosome Changes Revealed by the Q-Band Staining Method during Cell Senescence of WI-38
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Q带染色法揭示WI-38细胞衰老过程中的染色体变化
DOI：
10.3181/00379727-147-38381
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发表时间：
1974
影响因子：
--
通讯作者：
F. Ruddle
中科院分区：
文献类型：
--
作者：
T. Chen;F. Ruddle
总结采用奎纳克林-芥子气荧光染料染色程序，在体外对人成纤维细胞系WI-38的早期、中期和晚期选定的中期细胞进行了Q带核型分析。未检测到染色体带型的系统性变化，表明染色体在衰老的所有阶段都是结构稳定的。因此可以得出结论，进行性染色体变化与衰老过程无关。然而，培养的WI-38成纤维细胞的染色体组成并不均匀的二倍体，如以前的报告所建议的。几个重复的染色体重排，即1 p-，4p-，和t（11 q-，17），检测到足够高的频率，以确保其产生的少数干细胞群体。还观察到干细胞群中的某些染色体重排，例如4p-，类似于与某些先天性异常相关的重排。可能这些染色体修饰优先出现和/或携带它们的细胞没有严重的不利条件。
Summary Employing the quinacrine-mustard flurochrome staining procedure, Q-band karyotypes were analysed in selected metaphases from early, middle, and late stages in vitro of a human fibroblastic line WI-38. Systematic shifts in the banding patterns of the chromosomes were not detected, suggesting that the chromosomes are structurally stable during all stages of senescence. It can thus be concluded that progressive chromosomal change is not a correlate of the aging process. However, the chromosome constitution of cultured WI-38 fibroblast is not uniformly diploid as suggested by previous reports. Several recurring chromosomal rearrangements, namely 1p-, 4p-, and t(11q-, 17), were detected at sufficiently high frequencies to insure their generation by minor stem cell populations. It was also observed that certain chromosomal rearrangements in the stem cell populations, as for example 4p-, resembled those associated with certain congenital abnormalities. Possibly these chromosomal modifications arise preferentially and/or the cells which bear them are not seriously disadvantaged.