The fate of neural progenitor cells expressing astrocytic and radial glial markers in the postnatal rat dentate gyrus
The fate of neural progenitor cells expressing astrocytic and radial glial markers in the postnatal rat dentate gyrus
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DOI：
10.1111/j.1460-9568.2005.04396.x
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发表时间：
2005-10-01
影响因子：
3.4
通讯作者：
Seki, T
中科院分区：
文献类型：
--
作者：
Namba, T;Mochizuki, H;Seki, T
在齿状回神经元继续产生从胚胎晚期到成人阶段。近年来的大量研究揭示了成体神经发生的几个关键方面，但对胚胎神经发生与成体神经发生之间的过渡阶段--出生后早期神经发生的研究却很少。在这里，我们专注于出生后早期的神经发生和检查的性质和发育的神经祖细胞在Wistar大鼠。Ki 67，细胞周期标志物，和5-溴-2-脱氧尿苷（BrdU）标记的免疫组织化学显示，细胞增殖主要发生在门，部分在颗粒下区。大多数增殖细胞表达S100 β和星形胶质细胞特异性谷氨酸转运蛋白（GLAST），并且亚群也对胶质细胞酸性蛋白（GFAP）和巢蛋白呈阳性。用BrdU和我们改进的携带增强型绿色荧光蛋白（GFP）的逆转录病毒载体进行追踪表明，大量增殖细胞在门部分化为增殖的神经母细胞和未成熟的神经元，然后迁移到颗粒细胞层（66.8%），在门部留下长的轴突样突起，颗粒下区主要为星形胶质细胞（12.0%）和放射状胶质样细胞（4.7%）。这些结果表明，表达星形胶质细胞和放射状胶质细胞标记物的颗粒细胞的祖细胞，增殖和分化成神经元主要在门在出生后早期。
In the dentate gyrus neurons continue to be generated from late embryonic to adult stage. Recent extensive studies have unveiled several key aspects of the adult neurogenesis, but only few attempts have so far been made on the analysis of the early postnatal neurogenenesis, a transition state between the embryonic and adult neurogenesis. Here, we focus on the early postnatal neurogenesis and examine the nature and development of neural progenitor cells in Wistar rats. Immunohistochemistry for Ki67, a cell cycle marker, and 5-bromo-2-deoxyuridine (BrdU) labelling show that cell proliferation occurs mainly in the hilus and partly in the subgranular zone. A majority of the proliferating cells express S100 beta and astrocyte-specific glutamate transporter (GLAST) and the subpopulation are also positive for glial fibrillary acidic protein (GFAP) and nestin. Tracing with BrdU and our modified retrovirus vector carrying enhanced green fluorescent protein (GFP) indicate that a substantial population of the proliferating cells differentiate into proliferative neuroblasts and immature neurons in the hilus, which then migrate to the granule cell layer (66.8%), leaving a long axon-like process behind in the hilus, and the others mainly become star-shaped astrocytes (12.0%) and radial glia-like cells (4.7%) in the subgranular zone. These results suggest that the progenitors of the granule cells expressing astrocytic and radial glial markers, proliferate and differentiate into neurons mainly in the hilus during the early postnatal period.