Linking membrane microdomains to the cytoskeleton: Regulation of the lateral mobility of reggie-1/flotillin-2 by interaction with actin
Linking membrane microdomains to the cytoskeleton: Regulation of the lateral mobility of reggie-1/flotillin-2 by interaction with actin
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DOI：
10.1016/j.febslet.2007.08.074
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发表时间：
2007-10-02
期刊：
影响因子：
3.5
通讯作者：
Stuermer, Claudia A. O.
中科院分区：
文献类型：
--
作者：
Langhorst, Matthias F.;Solis, Gonzalo P.;Stuermer, Claudia A. O.
REGES/FLOTLINS是膜微区的寡聚体支架蛋白。我们在这里展示了Reggie-1/Flotillin-2微域沿着皮质F-肌动蛋白在几种细胞类型中组织。体内共定位和体外结合实验表明，与F-肌动蛋白的相互作用是由SPFH结构域介导的。FRAP显示，Reggie-1/Flotillin-2微域的形成独立于肌动蛋白，但肌动蛋白细胞骨架的破坏或稳定调节了Reggie-1/Flotillin-2的侧向迁移率。此外，通过肌动蛋白聚合可以有效地固定Reggie/Flotillin微域，而通过TIRF显微镜对单个微域的跟踪表明，肌动蛋白破坏可以促进微域之间的Reggie-1/Flotillin-2分子交换。(C)2007年欧洲生化学会联合会。爱思唯尔出版，版权所有。
The reggies/flotillins are oligomeric scaffolding proteins for membrane microdomains. We show here that reggie-1/flotillin-2 microdomains are organized along cortical F-actin in several cell types. Interaction with F-actin is mediated by the SPFH domain as shown by in vivo co-localization and in vitro binding experiments. Reggie-1/flotillin-2 microdomains form independent of actin, but disruption or stabilization of the actin cytoskeleton modulate the lateral mobility of reggie-1/flotillin-2 as shown by FRAP. Furthermore, reggie/flotillin microdomains can efficiently be immobilized by actin polymerisation, while exchange of reggie-1/flotillin-2 molecules between microdomains is enhanced by actin disruption as shown by tracking of individual microdomains using TIRF microscopy. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.