The circular F-actin bundles provide a track for turnaround and bidirectional movement of mitochondria in Arabidopsis root hair.
The circular F-actin bundles provide a track for turnaround and bidirectional movement of mitochondria in Arabidopsis root hair.
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DOI：
10.1371/journal.pone.0091501
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Zhang Y;Sheng X;Meng X;Li Y
根毛中细胞器的运动主要是沿着肌动蛋白细胞骨架进行的。植物根毛细胞中的细胞器（如线粒体和高尔基体）进行双向运动的典型形式是循环和“反向喷泉”细胞质流动。然而，目前还缺乏深入的研究，关于肌动蛋白细胞骨架的分布和周转细胞器运动之间的关系，在植物根毛细胞。本文以GFP-ABD 2-GFP（绿色荧光蛋白-肌动蛋白结合域2-绿色荧光蛋白）稳定转化的拟南芥幼苗为材料，研究了丝状肌动蛋白束在根毛中的分布以及线粒体沿着这些纤维束的定向运动。激光扫描共聚焦显微镜观察发现，拟南芥根的表皮细胞和根毛中广泛存在环状的F-肌动蛋白束。在根毛中，这些环形束主要开始于亚顶端区域，这是细胞器转向运动发生的位置。用MitoTracker探针标记线粒体，激光共聚焦扫描显微镜动态观察发现，线粒体沿沿着环形F-肌动蛋白束发生转向运动。相关实验结果表明，环状的F-肌动蛋白束为线粒体的转向和双向运动提供了一个轨道。
The movement of organelles in root hairs primarily occurs along the actin cytoskeleton. Circulation and “reverse fountain” cytoplasmic streaming constitute the typical forms by which most organelles (such as mitochondria and the Golgi apparatus) in plant root hair cells engage in bidirectional movement. However, there remains a lack of in-depth research regarding the relationship between the distribution of the actin cytoskeleton and turnaround organelle movement in plant root hair cells. In this paper, Arabidopsis seedlings that had been stably transformed with a GFP-ABD2-GFP (green fluorescent protein-actin-binding domain 2-green fluorescent protein) construct were utilized to study the distribution of bundles of filamentous (F)-actin and the directed motion of mitochondria along these bundles in root hairs. Observations with a confocal laser scanning microscope revealed that there were widespread circular F-actin bundles in the epidermal cells and root hairs of Arabidopsis roots. In root hairs, these circular bundles primarily start at the sub-apical region, which is the location where the turnaround movement of organelles occurs. MitoTracker probes were used to label mitochondria, and the dynamic observation of root hair cells with a confocal laser scanning microscope indicated that turnaround mitochondrial movement occurred along circular F-actin bundles. Relevant experimental results demonstrated that the circular F-actin bundles provide a track for the turnaround and bidirectional movement of mitochondria.
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