Increased glyceraldehyde-3-phosphate dehydrogenase gene expression in human pancreatic adenocarcinoma.
Increased glyceraldehyde-3-phosphate dehydrogenase gene expression in human pancreatic adenocarcinoma.
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发表时间：
1988-11
期刊：
影响因子：
11.2
通讯作者：
Nancy Schek;Bruce Lee Hall;O. Finn
中科院分区：
文献类型：
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作者：
Nancy Schek;Bruce Lee Hall;O. Finn
为了鉴定和表征基因，其产物在胰腺癌中发挥作用，我们使用来自胰腺癌细胞系HPAF的mRNA构建了互补DNA（cDNA）文库，所述胰腺癌细胞系HPAF作为裸鼠肿瘤生长。通过差异筛选，我们确定了一个cDNA克隆，pII 5 B，这是同源的mRNA表达在显着更高的水平在HPAF细胞比正常人胰腺。pII 5 B cDNA与甘油醛-3-磷酸脱氢酶（GAPDH，EC 1.2.1.12）mRNA的3 ′-非翻译区同源。几个HPAF肿瘤GAPDH cDNA克隆的部分测序显示与先前公布的GAPDH cDNA序列没有显着差异。与正常胰腺相比，在6个胰腺癌细胞系和2个裸鼠肿瘤中发现GAPDH mRNA水平相对于肌动蛋白mRNA水平增加。烯醇化酶和葡萄糖转运蛋白的mRNA水平也在HPAF细胞和裸鼠肿瘤中增加，表明胰腺癌中糖酵解相关基因的表达普遍增加。与正常胰腺相比，GAPDH蛋白在裸鼠肿瘤和新鲜人胰腺癌中的水平升高。高GAPDH水平可能是人腺癌的特征，因为结肠腺癌与正常结肠相比也表现出高水平的GAPDH。
To identify and characterize genes, the products of which play a role in pancreatic adenocarcinoma, we constructed a complementary DNA (cDNA) library using mRNA from the pancreatic adenocarcinoma cell line HPAF, grown as a nude mouse tumor. Through differential screening, we identified a cDNA clone, pII5B, that is homologous to an mRNA expressed at significantly higher levels in HPAF cells than in normal human pancreas. The pII5B cDNA was homologous to the 3'-untranslated region of glyceraldehyde-3-phosphate dehydrogenase (GAPDH, EC 1.2.1.12)mRNA. Partial sequencing of several HPAF tumor GAPDH cDNA clones revealed no significant differences from previously published GAPDH cDNA sequences. Increased levels of GAPDH mRNA, relative to actin mRNA levels, were found in six pancreatic adenocarcinoma cell lines and two nude mouse tumors, when compared to normal pancreas. Enolase and glucose transporter mRNA levels were also increased in HPAF cells and nude mouse tumor, suggesting a general increase in expression of genes associated with glycolysis in pancreatic adenocarcinoma. Levels of GAPDH protein were elevated in nude mouse tumors and fresh human pancreatic adenocarcinomas compared to normal pancreas. High GAPDH levels may be characteristic of human adenocarcinomas, since colon adenocarcinomas also exhibited high levels of GAPDH compared to normal colon.