Reichenbachiella versicolor sp nov., isolated from red alga
Reichenbachiella versicolor sp nov., isolated from red alga
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Reichenbachiella versicolor sp nov.，从红藻中分离出来
DOI：
10.1099/ijsem.0.003023
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发表时间：
2018
影响因子：
2.8
通讯作者：
Du Zong-Jun
中科院分区：
文献类型：
--
作者：
Shi Ming-Jing;Wang Chong;Liu Zuan-Yan;Jiang Lai-Xiang;Du Zong-Jun
从采自中国海南省陵水县沿海的红藻门藻类Gracilariablodgettii中分离到一株革兰氏染色阴性、严格好氧、无鞭毛、棒状细菌菌株，命名为DC 003 T。该菌株在28 °C、pH 7.0-7.5和2.0- 3.0%（w/v）NaCl存在下生长最佳。 根据16 S rRNA基因序列分析，菌株DC 003 T属于Reichenbachiella属，与Reichenbachiella agariperforans JCM 11238的亲缘关系最近（94.5%），其次是Reichenbachiella faecimodium JCM 16588 T（94.2%）。 主要呼吸醌为甲基萘醌7，主要脂肪酸为异C15：0和总特征3（C16：1ω 7 c和/或异C15：02-OH）。 极性脂质由磷脂酰乙醇胺、两种未鉴定的氨基磷脂、三种未鉴定的磷脂和10种未鉴定的脂质组成。基因组DNA的G+C含量为37.1mol%。根据表型、基因型和系统发育分析，DC 003 T菌株被认为是赖氏酵母属的一个新种，命名为赖氏酵母属（Reichenbachiellaversicolorsp.）十一是建议。模式菌株为DC 003 T（=KCTC 42867 T =MCCC 1H 00130 T）。
A Gram-stain-negative, strictly aerobic, non-flagellated, rod-shaped bacterial strain, designated DC003T, was isolated from the algaGracilariablodgettiiof the phylumRhodophytacollected from the coast of Lingshui county, Hainan, China. The strain grew optimally at 28 °C, pH 7.0–7.5 and in the presence of 2.0–3.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed strain DC003Tto be within the genusReichenbachiella, and most closely related toReichenbachiella agariperforansJCM11238 (94.5 %), followed byReichenbachiella faecimarisJCM 16588T(94.2 %). The major respiratory quinone was menaquinone 7 and the major fatty acids were iso-C15 : 0and summed feature 3 (C16 : 1ω7cand/or iso-C15 : 02-OH). The polar lipids consisted of phosphatidylethanolamine, two unidentified aminophospholipids, three unidentified phospholipids and 10 unidentified lipids. The G+C content of the genomic DNA was 37.1 mol%. On the basis of the phenotypic, genotypic and phylogenetic analysis, strain DC003Tis considered to represent a novel species of the genusReichenbachiella, for which the nameReichenbachiellaversicolorsp. nov. is proposed. The type strain is DC003T(=KCTC 42867T=MCCC 1H00130T).