Number and spatial distribution of intrinsically photosensitive retinal ganglion cells in the adult albino rat
Number and spatial distribution of intrinsically photosensitive retinal ganglion cells in the adult albino rat
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DOI：
10.1016/j.exer.2012.12.010
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发表时间：
2013-03-01
影响因子：
3.4
通讯作者：
Vidal-Sanz, M.
中科院分区：
文献类型：
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作者：
Galindo-Romero, C.;Jimenez-Lopez, M.;Vidal-Sanz, M.
视网膜内光敏神经节细胞（ipRGC）直接对光作出反应，并负责昼夜节律与光刺激的同步和瞳孔光反射。为了量化大鼠ipRGC的总人口并评估其空间分布，我们开发了一种自动化程序并使用相邻地图。此外，在所有分析的视网膜中，我们研究了RGC的一般群体（通过其Brn3a表达鉴定）和ipRGC的群体（通过黑视素免疫检测鉴定），从而允许对其形貌进行共分析。我们的结果显示，白化病大鼠中ipRGC的总平均数+/-标准差为2047 +/-309。它们在视网膜中的分布似乎与Brn3a（+）RGC的分布互补，在周边更密集，特别是在上级视网膜中，在视条纹上方的颞象限中发现它们的最高密度。此外，通过追踪来自两个上级丘的视网膜，我们还确定了90.62%的ipRGC投射到这些中央靶标。(C)2012爱思唯尔有限公司保留所有权利。
Intrinsically photosensitive retinal ganglion cells (ipRGCs) respond directly to light and are responsible of the synchronization of the circadian rhythm with the photic stimulus and for the pupillary light reflex. To quantify the total population of rat-ipRGCs and to assess their spatial distribution we have developed an automated routine and used neighbour maps. Moreover, in all analysed retinas we have studied the general population of RGCs - identified by their Brn3a expression - and the population of ipRGCs - identified by melanopsin immunodetection - thus allowing the co-analysis of their topography. Our results show that the total mean number +/- standard deviation of ipRGCs in the albino rat is 2047 +/- 309. Their distribution in the retina seems to be complementary to that of Brn3a(+)RGCs, being denser in the periphery, especially in the superior retina where their highest densities are found in the temporal quadrant, above the visual streak. In addition, by tracing the retinas from both superior colliculi, we have also determined that 90.62% of the ipRGC project to these central targets. (C) 2012 Elsevier Ltd. All rights reserved.