Quantitative ocular bacteriology: a method for the enumeration and identification of bacteria from the skin-lash margin and conjunctiva.
Quantitative ocular bacteriology: a method for the enumeration and identification of bacteria from the skin-lash margin and conjunctiva.
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眼部细菌定量学：一种对皮肤睫毛边缘和结膜细菌进行计数和鉴定的方法。
DOI：
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发表时间：
1981
影响因子：
4.4
通讯作者：
R. Abshire
中科院分区：
文献类型：
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作者：
G. Cagle;R. Abshire
使用1型Calgiswabs从正常志愿者的皮肤睫毛边缘和结膜收集细菌标本。在运输至实验室期间，拭子保存在非营养平衡盐溶液中。藻酸盐拭子以两步程序溶解在TC Eagle培养基和2.5%六偏磷酸钠溶液中。将来自溶解拭子的悬浮细菌的等分试样涂布在血琼脂上。孵育后，对存在的细菌菌落进行计数和鉴定。从皮肤睫毛边缘和结膜培养显示分离的微生物的数量和类型的显着变化。从睑缘分离出的细菌数通常大于结膜。表皮葡萄球菌是分离的最普遍的细菌。藻酸盐拭子从细菌悬浮液中吸附细菌和从先前接种的兔眼结膜中回收产生红色素的生物体表明该方法灵敏且重现性高。
Type 1 Calgiswabs were used to collect bacterial specimens from the skin-lash margins and conjunctivae of normal volunteers. The swabs were held in a nonnutritive balanced salt solution during transportation to the laboratory. Alginate swabs were dissolved in a two-step procedure in TC Eagle's medium and 2.5% sodium hexametaphosphate solution. Aliquots of suspended bacteria from the dissolved swab were plated on blood agar. After incubation, bacterial colonies present were enumerated and identified. Cultures from both the skin-lash margin and conjunctiva show significant variation in the number and types of microorganisms isolated. Quantities of bacteria isolated from the lid margin were usually greater than from the conjunctiva. Staphylococcus epidermidis was the most prevalent bacterium isolated. The adsorption of bacteria onto alginate swabs from bacterial suspensions and the recovery of a red pigment-producing organism from the conjunctivae of rabbit eyes previously inoculated show that the method is sensitive and highly reproducible.