Expression and localization of aquaporins, members of the water channel family, during development of the rat submandibular gland
Expression and localization of aquaporins, members of the water channel family, during development of the rat submandibular gland
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DOI：
10.1007/s00424-003-1109-9
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发表时间：
2003-09-01
影响因子：
4.5
通讯作者：
Hosoi, K
中科院分区：
文献类型：
--
作者：
Akamatsu, T;Parvin, MN;Hosoi, K
应用RT-PCR、北方印迹和免疫组织化学方法研究水通道蛋白家族成员水通道蛋白（AQP 1-AQP 5）在大鼠颌下腺发育过程中的表达和定位，探讨其与颌下腺发育的关系。RT-PCR分析显示每个AQP的独特表达模式。AQP 1在胚胎发育过程中呈组成型表达，而AQP 5在胚胎16.5天（E16）至E20的发育过程中表达增强。这些表达模式与北方印迹分析的结果一致。在北方印迹中未检测到AQP 3和AQP 4 mRNA，但在RT-PCR中检测到它们。在出生后的发育过程中，AQP 5和AQP 1的mRNA持续表达，但没有检测到AQP 3或AQP 4的信息。AQP 2 mRNA在出生前或出生后的发育过程中，在该组织中未检测到。免疫组化结果显示，AQP 5在E18时首先定位于前腺泡细胞的顶膜，随着成熟腺泡的分化和建立，AQP 5在腺泡细胞的顶膜上也有明显的分布。此外，一些血管也表现出AQP 5的免疫反应性。AQP 1在整个发育过程中免疫定位于腺体的血管，包括毛细血管。这些观察结果表明，存在的转录调节大鼠AQP 5，这是一个最有可能的调节唾液的产生和分泌，在建立功能的颌下腺。
The expression and localization of aquaporins (AQP1-AQP5), members of the water channel family, in the developing rat submandibular gland were analysed using RT-PCR, Northern blotting and immunohistochemistry to explore their relation to the development of this salivary gland. RT-PCR analysis revealed unique expression patterns of each AQP. AQP1 was expressed constitutively during prenatal development, whereas the expression of AQP5 became more intense in the course of development from embryonic day 16.5 (E16) to E20. These expression patterns concurred with the results of Northern blot analysis. AQP3 and AQP4 mRNAs in the prenatal development were not detected in Northern blots, although they were detected by RT-PCR. During postnatal development, AQP5 and AQP1 mRNAs were expressed continuously, but no message for AQP3 or AQP4 was detected. AQP2 mRNA was not detected during either prenatal or postnatal development in this tissue. Immunohistochemical studies revealed that AQP5 was first localized at the apical membrane of proacinar cells at E18, and then became clearly distributed at the apical membrane of acinar cells in accordance with the differentiation and establishment of the mature acini. In addition, some vasculature also showed immunoreactivity for AQP5. AQP1 was immunolocalized in the blood vessels, including capillaries, of the gland throughout development. These observations suggest the existence of transcriptional regulation of rat AQP5, which is one of the most probable regulators of saliva production and secretion, during the establishment of the functional submandibular salivary gland.