SPIN-TRAPPING AND DIRECT ELECTRON-SPIN RESONANCE INVESTIGATIONS OF THE REDOX METABOLISM OF QUINONE ANTI-CANCER DRUGS
SPIN-TRAPPING AND DIRECT ELECTRON-SPIN RESONANCE INVESTIGATIONS OF THE REDOX METABOLISM OF QUINONE ANTI-CANCER DRUGS
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DOI：
10.1016/0304-4165(80)90142-7
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
MASON, RP
中科院分区：
文献类型：
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作者：
KALYANARAMAN, B;PEREZREYES, E;MASON, RP
在含有阿霉素、柔红霉素和丝裂霉素C的[大鼠肝]微粒体孵育液中自旋捕获超氧自由基。这些含醌抗癌药物的自旋捕获超氧化物和半醌自由基代谢物出现的时间顺序表明，半醌的空气氧化是超氧化物形成的原因。超氧化物歧化酶阻止超氧化物自旋加合物的形成。含有蒽环类药物嵌入DNA的微粒体孵育产生的超氧化物比无DNA的孵育少得多。丝裂霉素C的半醌代谢物在生物系统中的第一个明确的ESR证据。
The superoxide free radical was spin trapped in [rat hepatic] microsomal incubations containing adriamycin, daunorubicin and mitomycin C. The time sequence of the appearance of the spin-trapped superoxide and the semiquinone radical metabolite of these quinone-containing anticancer drugs indicates that air oxidation of the semiquinone is responsible for the superoxide formation. Superoxide dismutase prevents the formation of the superoxide spin adducts. Microsomal incubations containing anthracyclines intercalated in DNA produce much less superoxide than incubations free of DNA. The first unambiguous ESR evidence for the semiquinone metabolite of mitomycin C in a biological system is presented.