Evolution and adaptation of H5N1 influenza virus in avian and human hosts in Indonesia and Vietnam
Evolution and adaptation of H5N1 influenza virus in avian and human hosts in Indonesia and Vietnam
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DOI：
10.1016/j.virol.2006.03.048
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发表时间：
2006-07-05
期刊：
影响因子：
3.7
通讯作者：
Guan, Y.
中科院分区：
文献类型：
--
作者：
Smith, G. J. D.;Naipospos, T. S. P.;Guan, Y.
高致病性禽流感病毒H5 N1在东亚和东南亚的家禽中流行，最近疾病暴发蔓延到中亚、欧洲和非洲的部分地区。据报道，在越南、泰国、柬埔寨、印度尼西亚和中国，人类继续受到种间传播，引起了大流行的担忧。在这里，我们的基因特征82 H5 N1病毒分离的家禽在整个印度尼西亚和越南和11人从越南南部的分离株与公共数据库中的序列数据，以解决有关的问题，病毒的引进，地方性和演变。系统发生分析显示，来自印度尼西亚的所有病毒都形成了H5 N1基因型Z病毒的独特亚系，这表明这次疫情可能起源于过去两年在全国传播的单一传入。印度尼西亚持续的病毒活动归因于该国境内家禽流动的传播，而不是通过鸟类迁徙的反复引入。在印度尼西亚和越南，H5 N1病毒随着时间的推移在每个国家内演变成地理上不同的群体。对Z基因型H5 N1病毒基因组的分子分析表明，只有M2和PB 1-F2基因受到正选择，这表明这些基因可能参与了该病毒在种间传播后对新宿主的适应。在氨基酸水平上，在HA和PB 1-F2蛋白中，在那些基因型Z病毒中有12个残基处于正选择下。其中一些残留物在人类分离株中比在禽类分离株中更常见，并且与病毒抗原性和受体结合有关。我们的研究提供了对H5 N1流感病毒正在进行的进化的深入了解，这些病毒在不同的鸟类物种中传播，并在鸟类和人类宿主之间的界面传播。(c)2006爱思唯尔公司All rights reserved.
Highly pathogenic avian influenza virus H5N1 is endemic in poultry in East and Southeast Asia with disease outbreaks recently spreading to parts of central Asia, Europe and Africa. Continued interspecies transmission to humans has been reported in Vietnam, Thailand, Cambodia, Indonesia and China, causing pandemic concern. Here, we genetically characterize 82 H5N1 viruses isolated from poultry throughout Indonesia and Vietnam and 11 human isolates from southern Vietnam together with sequence data available in public databases to address questions relevant to virus introduction, endemicity and evolution. Phylogenetic analysis shows that all viruses from Indonesia form a distinct sublineage of H5N1 genotype Z viruses suggesting this outbreak likely originated from a single introduction that spread throughout the country during the past two years. Continued virus activities in Indonesia were attributed to transmission via poultry movement within the country rather than through repeated introductions by bird migration. Within Indonesia and Vietnam, H5N1 viruses have evolved over time into geographically distinct groups within each country. Molecular analysis of the H5N1 genotype Z genome shows that only the M2 and PB1-F2 genes were under positive selection, suggesting that these genes might be involved in adaptation of this virus to new hosts following interspecies transmission. At the amino acid level 12 residues were under positive selection in those genotype Z viruses, in the HA and PB1-F2 proteins. Some of these residues were more frequently observed in human isolates than in avian isolates and are related to viral antigenicity and receptor binding. Our study provides insight into the ongoing evolution of H5N1 influenza viruses that are transmitting in diverse avian species and at the interface between avian and human hosts. (c) 2006 Elsevier Inc. All rights reserved.