Effect of in feed administration of different butyrate formulations on Salmonella Enteritidis colonization and cecal microbiota in broilers
Effect of in feed administration of different butyrate formulations on Salmonella Enteritidis colonization and cecal microbiota in broilers
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DOI：
10.1186/s13567-020-00780-2
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发表时间：
2020-04-19
影响因子：
4.4
通讯作者：
Van Immerseel, Filip
中科院分区：
文献类型：
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作者：
Onrust, Lonneke;Baeyen, Steve;Van Immerseel, Filip
丁酸盐已被广泛用作饲料添加剂，以改善肠道健康和减少沙门氏菌在家禽中的定植。沙门氏菌主要定植在盲肠，因此丁酸盐浓度应在该肠段中增加。因此，在科学文献中描述的丁酸盐对沙门氏菌定植的影响的不确定性可能是由于丁酸盐释放位置效应。在本研究中，评价了新开发的丁酸盐制剂对盲肠丁酸盐浓度和肠道沙门氏菌定植的影响。在第一次试验中，将肉鸡随机分配到7个饮食处理组，其具有基于不同方法的制剂以改变丁酸盐释放曲线：基于扩散/侵蚀从蜡基质释放;微丸假定在结肠中约pH 7释放丁酸盐;三丁酸甘油酯基于小肠中酯的水解。脂肪保护的丁酸盐被包括作为参考，因为其已知对减少沙门氏菌定植的作用。感染后4天，测定每g盲肠内容物和脾脏中的cfu沙门氏菌数。蜡基质中的丁酸盐制剂显著降低了盲肠内容物中的沙门氏菌定植。在第二次试验中，蜡和脂肪保护的丁酸盐处理被重复，并且第一次试验的结果被证实。与对照组相比，对于沙门氏菌定植减少的那些组，在盲肠中观察到更高比例的丁酸盐浓度。这与盲肠微生物群的有益转变有关。总之，增加盲肠丁酸盐浓度的制剂在防止沙门氏菌肠杆菌定植方面是上级的。
Butyrate has been used extensively as a feed additive to improve gut health and to decrease Salmonella colonization in poultry. Salmonella mainly colonizes the ceca so butyrate concentrations should be increased in this gut segment. Discrepancies on the effects of butyrate on Salmonella colonization, described in the scientific literature, could thus be due to butyrate release location effects. In this study, newly developed butyrate formulations were evaluated for their effect on cecal butyrate concentrations and on colonization by Salmonella Enteritidis. In a first trial, broilers were randomly allocated to 7 dietary treatment groups with formulations based on different approaches to modify the butyrate release profile: release from wax matrices based on diffusion/erosion; micropellets supposedly release butyrate around pH 7 in the colon; tributyrin is based on the hydrolysis of esters in the small intestine. Fat-protected butyrate was included as a reference, because of its known effect on reduction of Salmonella colonization. Four days after infection, the number of cfu Salmonella per g cecal content and spleen were determined. Butyrate formulations in a wax matrix significantly reduced the Salmonella colonization in cecal content. In a second trial, wax and fat-protected butyrate treatments were replicated and results from the first trial were confirmed. Compared to the control group a higher proportion of butyrate concentration was observed in ceca for those groups with reduced Salmonella colonization. This was associated with a beneficial shift in the cecal microbiota. In conclusion, formulations that increase cecal butyrate concentrations are superior in protecting against Salmonella Enteritidis colonization.