Influence of genotype and texture on zein content in endosperm of maize grains
Influence of genotype and texture on zein content in endosperm of maize grains
复制标题
基因型和质地对玉米籽粒胚乳玉米醇溶蛋白含量的影响
DOI：
10.1111/j.1744-7348.2007.00173.x
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
S. Delhaye
中科院分区：
文献类型：
--
作者：
J. Landry;S. Delhaye
基因型和质地对玉米籽粒蛋白质含量的影响进行了研究，通过评估的绝对量的6个蛋白质组分的全胚乳的4个野生型系高蛋白含量和4个优质蛋白玉米（QPM）品种和手工解剖的硬和软胚乳区域从其他8行。如先前报道的6个野生型品系及其opaque-2（o2）版本，玉米醇溶蛋白在所有遗传背景和所有类型的胚乳中占主导地位。从这些数据和其他数据中，野生型品系的发育和成熟胚乳中的玉米醇溶蛋白和真蛋白（不含非蛋白氮的粗蛋白）的量是相关的。来自硬胚乳的玉米醇溶蛋白的数据点位于回归线和置信区上限之间。来自软胚乳的玉米醇溶蛋白位于置信区的下部，与最不成熟胚乳对应的点处于同一水平。此外，来自o2和QPM样品的玉米醇溶蛋白的一些数据点位于下限附近，而其他数据点在置信区域之外。这表明玉米醇溶蛋白的初始积累依赖于基因型，以较低的相对速率，随后以较高的速率积累。讨论了玉米醇溶蛋白的分离和定量条件。
The effect of genotypes and texture on the content of proteins in maize grains was examined by assessing absolute amounts of six protein fractions in the whole endosperms of four wild-type lines with high protein content and four quality protein maize (QPM) varieties and for hand-dissected hard and soft endosperm regions from eight other lines. As previously reported for six wild-type lines and their opaque-2 (o2) versions, zeins were predominant for all genetic backgrounds and all types of endosperms. From these data and others the amounts of zeins and true proteins (crude proteins free of non-protein nitrogen) in developing and mature endosperms of wild-type lines were correlated. The data points for zeins from hard endosperms lay between the regression line and the upper limit of confidence area. Those for zeins from soft endosperms were located at the lower part of confidence area and on a level with the points corresponding to the most immature endosperms. Furthermore, some data points for zeins from o2 and QPM samples lay near the lower limit while the others were outside the confidence area. This suggested an initial zein accumulation dependent on the genotype at a low relative rate, followed by an accumulation at higher rate. The conditions used for isolating and quantitating zeins are discussed.