EGG-3 regulates cell-surface and cortex rearrangements during egg activation in Caenorhabditis elegans
EGG-3 regulates cell-surface and cortex rearrangements during egg activation in Caenorhabditis elegans
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DOI：
10.1016/j.cub.2007.08.011
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发表时间：
2007-09-18
期刊：
影响因子：
9.2
通讯作者：
Singson, Andrew
中科院分区：
文献类型：
--
作者：
Maruyama, Rika;Velarde, Nathalie V.;Singson, Andrew
受精触发卵子激活并将卵子转化为发育中的胚胎。这种卵-胚胎转变的事件通常包括减数分裂的恢复、皮质肌动蛋白细胞骨架的重组和卵母细胞表面的重塑[1-3]。调节精子依赖性卵子激活事件的因素还不清楚。秀丽隐杆线虫EGG-3是蛋白酪氨酸磷酸酶样（PTPL）家族的成员[4]，在响应精子进入的卵子激活期间调节细胞表面和皮质重排是必需的。虽然受精正常发生在卵-3突变体，F-肌动蛋白的极化分散被改变，几丁质蛋壳没有形成，没有极体产生。EGG-3以类似于CHS-1和MBK-2的模式与卵母细胞质膜相关。CHS-1是蛋壳沉积所必需的[5-7]，而MBK-2是卵-胚胎过渡期间母体蛋白降解所必需的[8-12]。CHS-1和EGG-3的定位是相互依赖的，这两个基因都是MBK-2在卵母细胞中正确定位所必需的。因此，EGG-3通过影响极化的F-肌动蛋白动力学和直接参与执行卵到胚胎转变的分子的定位或活性在卵激活中起着核心作用。
Fertilization triggers egg activation and converts the egg into a developing embryo. The events of this egg-to-embryo transition typically include the resumption of meiosis, the reorganization of the cortical actin cytoskeleton, and the remodeling of the oocyte surface [1-3]. The factors that regulate sperm-dependent egg-activation events are not well understood. Caenorhabditis elegans EGG-3, a member of the protein tyrosine phosphatase-like (PTPL) family [4], is essential for regulating cell-surface and cortex rearrangements during egg activation in response to sperm entry. Although fertilization occurred normally in egg-3 mutants, the polarized dispersal of F-actin is altered, a chitin eggshell is not formed, and no polar bodies are produced. EGG-3 is associated with the oocyte plasma membrane in a pattern that is similar to CHS-1 and MBK-2. CHS-1 is required for eggshell deposition [5-7], whereas MBK-2 is required for the degradation of maternal proteins during the egg-to-embryo transition [8-12]. The localization of CHS-1 and EGG-3 are interdependent and both genes were required for the proper localization of MBK-2 in oocytes. Therefore, EGG-3 plays a central role in egg activation by influencing polarized F-actin dynamics and the localization or activity of molecules that are directly involved in executing the egg-to-embryo transition.