Comparative Analysis of Sporulating and Spore-Deficient Strains of Agrocybe salicacola Based on the Transcriptome Sequences
Comparative Analysis of Sporulating and Spore-Deficient Strains of Agrocybe salicacola Based on the Transcriptome Sequences
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基于转录组序列的杨树菇产孢菌株和孢子缺陷菌株的比较分析
DOI：
10.1007/s00284-015-0819-5
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发表时间：
2015-08-01
影响因子：
2.6
通讯作者：
Zhao, Yong-Chang
中科院分区：
文献类型：
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作者：
Chen, Wei-Min;Zhang, Xiao-Lei;Zhao, Yong-Chang
食用菌产生的大量孢子会引起许多问题，包括引起肺部疾病，消耗天然遗传多样性，以及降低子实体的质量。获得芽孢缺陷型菌株并了解这些菌株的潜在分子机制对于育种工作非常重要。本研究以食用菌柳生茶树菇（Agrocybe salicacola）的单核菌株为材料，通过杂交获得了3个孢子缺失的菌株。交配试验表明，在有丝分裂期或减数分裂期，一些菌株中存在的隐性等位基因可能控制着小梗的发育。转录组分析表明，大多数参与DNA错配修复，碱基切除修复和同源重组的基因在突变子实体中表现出下调的表达模式。与GTP环化水解酶编码基因、DNA修复基因rad 8、细胞壁完整性和应激反应组分编码基因高度相似的5个基因片段均在野生型菌株中特异表达，为研究食用菌孢子发育提供了重要信息。
The large number of spores produced by edible mushrooms cause many problems, including causing lung disease, depleting natural genetic diversity, and reduced quality of fruiting bodies. Obtaining spore-deficient strains and understanding the underlying molecular mechanisms of such strains are important for breeding work. In this study, we crossed monokaryotic strains isolated from the edible fungi Agrocybe salicacola to obtain three spore-deficient strains with losses of the sterigmata on the surface of the lamella. A mating test revealed that recessive alleles distributed in some strains might control sterigmata development during the mitotic or meiotic phases. Transcriptome analysis revealed that the majority of the genes involved in DNA mismatch repair, base excision repair, and homologous recombination exhibited down-regulated expression patterns in the mutant fruiting bodies. Five genetic fragments, which were highly similar to the GTP-cyclohydrolase encoding gene, the DNA repair gene rad 8, and cell wall integrity and stress response component-encoding genes, were all expressed exclusively in the wild-type strains; these findings provide important information for the study of the spore development of edible fungi.