Identification and characterization of human HESL, rat Hesl and rainbow trout hesl genes in silico.
Identification and characterization of human HESL, rat Hesl and rainbow trout hesl genes in silico.
复制标题
人类 HESL、大鼠 Hesl 和虹鳟鱼 hesl 基因的计算机识别和表征。
DOI：
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发表时间：
2004
影响因子：
5.4
通讯作者：
M. Katoh
中科院分区：
文献类型：
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作者：
Masuko Katoh;M. Katoh
Notch信号通路的激活导致Notch胞内结构域（NIC）的核转位，并通过CSL蛋白（RBPSUH或RBPSUHL）与NIC之间的相互作用激活靶基因的转录。HES 1、HES 5、HEY 1和HEY 2是Notch靶基因。哺乳动物HES/HEY家族蛋白以及果蝇Hairy和分裂增强子与细胞命运决定有关。我们先前已经鉴定和表征了人HES 2、HES 3和HES 5基因。在这里，我们确定和特征在于人类HES样（HESL），大鼠Fesl，虹鳟鱼Fesl基因，通过使用生物信息学。HESL基因位于AC093824.3基因组序列中，定位于人类染色体4q35.1，位于ACSL 1和SLC 25 A4基因之间。大鼠Fesl基因定位于大鼠染色体16 q11，位于AC111303.4基因组序列内。EST BX 875070.2是虹鳟fesl的代表性cDNA。HESL-ACSL 1位点在人、大鼠、小鼠和斑马鱼基因组中是保守的。人HESL（241 aa）与大鼠Hesl（240 aa）显示92.9%的总氨基酸同一性，与小鼠Hesl（240 aa）显示92.1%的总氨基酸同一性，并且与虹鳟鱼Hesl（235 aa）显示68.0%的总氨基酸同一性。HESL直系同源物由碱性螺旋-环-螺旋（bHLH）结构域和橙子结构域组成。HESL直系同源物的C-末端区域不同于HES、HEY和DEC同源物的C-末端区域。系统发育分析表明，具有橙子结构域的bHLH转录因子可分为3组：（I）HES 1、HES 2、HES 4和HES 6同源物;（II）HESL、HES 5、HES 7、HEY 1、HEY 2和HEYL同源物;（III）HES 3、DEC 1/BHLHB 2和DEC 2/BHLHB 3同源物。
Activation of Notch signaling pathway leads to nuclear translocation of Notch intracellular domain (NIC), and transcriptional activation of target genes through the interaction between CSL proteins (RBPSUH or RBPSUHL) and NIC. HES1, HES5, HEY1 and HEY2 are Notch target genes. Mammalian HES/HEY family proteins as well as Drosophila Hairy and Enhancer of split are implicated in the cell fate determination. We have previously identified and characterized human HES2, HES3 and HES5 genes. Here, we identified and characterized human HES-like (HESL), rat Fesl, and rainbow trout fesl genes by using bioinformatics. Human HESL gene, located within AC093824.3 genome sequence, was mapped to human chromosome 4q35.1 between ACSL1 and SLC25A4 genes. Rat Fesl gene, located within AC111303.4 genome sequence, was mapped to rat chromosome 16q11. EST BX875070.2 was the representative rainbow trout fesl cDNA. HESL-ACSL1 locus was conserved among human, rat, mouse, and zebrafish genomes. Human HESL (241 aa) showed 92.9% total-amino-acid identity with rat Hesl (240 aa), 92.1% total-amino-acid identity with mouse Hesl (240 aa), and 68.0% total-amino-acid identity with rainbow trout hesl (235 aa). HESL orthologs consist of basic Helix-loop-helix (bHLH) domain and ORANGE domain. C-terminal region of HESL orthologs were divergent from that of HES, HEY, and DEC homologs. Phylogenetic analyses revealed that bHLH transcription factors with ORANGE domain were classified into the following three groups: (group I) HES1, HES2, HES4 and HES6 orthologs; (group II) HESL, HES5, HES7, HEY1, HEY2 and HEYL orthologs; (group III) HES3, DEC1/BHLHB2 and DEC2/BHLHB3 orthologs.