Study of single and multidigit activation in monkey somatosensory cortex using voltage-sensitive dye imaging.
Study of single and multidigit activation in monkey somatosensory cortex using voltage-sensitive dye imaging.
复制标题
使用电压敏感染料成像研究猴子体感皮层的单指和多指激活
DOI：
10.1117/1.nph.4.3.031219
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发表时间：
2017-07
期刊：
影响因子：
5.3
通讯作者：
Friedman RM
中科院分区：
文献类型：
--
作者：
Roe AW;Winberry JE;Friedman RM
摘要。为了更好地理解手操作过程中的皮肤感觉统合，我们采用电压敏感染料（VSD）成像技术研究了麻醉猴子初级体感皮层（SI）在单指和多指触觉刺激下的组织和动态变化。我们发现在猕猴和松鼠猴的SI中，VSD显示了与先前电生理和固有信号成像研究一致的清晰的病灶手指地形。VSD还揭示了SI对多指刺激的反应。在松鼠猴3b和1区的触觉漏斗范式中，VSD显示阈下影响的两位数诱导，与外侧皮质内抑制一致。在触觉表观运动刺激下，VSD区域1和3b对运动刺激的反应优于静态触觉刺激。不同手指位置对“朝向手指”刺激的反应比较表明，1区存在方向选择反应；然而，还需要进一步的研究。这些令人兴奋的结果表明，VSD是研究非人类灵长类动物体感觉皮层加工的有力工具，并应进一步发展为未来清醒行为猴子体感觉研究。
Abstract. Toward the goal of understanding cutaneous sensory integration during manual behavior, we used voltage-sensitive dye (VSD) imaging to study the organization and dynamics of anesthetized monkey primary somatosensory cortex (SI) in response to single and multidigit tactile stimulation. We find that in both macaque and squirrel monkey SI, VSD reveals clear focal digit topography consistent with previous electrophysiological and intrinsic signal imaging studies. VSD also reveals interactions in SI in response to multidigit stimulation. With a tactile funneling paradigm in areas 3b and 1 in squirrel monkeys, VSD reveals two-digit induction of subthreshhold influences, consistent with lateral intracortical inhibition. In response to tactile apparent motion stimuli, VSD reveals preferential response to motion stimuli over static tactile stimuli in both areas 1 and 3b. Comparison of the response at different digit locations to “toward digit” stimuli suggests the presence of direction-selective response in area 1; however, further study is needed. These exciting results indicate that VSD constitutes a powerful tool for studying somatosensory cortical processing in nonhuman primates and should be further developed for future somatosensory studies in awake behaving monkeys.