The Gram-negative phytopathogen Xanthomonas campestris pv. campestris employs a 5'UTR as a feedback controller to regulate methionine biosynthesis.
The Gram-negative phytopathogen Xanthomonas campestris pv. campestris employs a 5'UTR as a feedback controller to regulate methionine biosynthesis.
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革兰氏阴性植物病原体野油菜黄单胞菌 pv。
DOI：
10.1099/mic.0.000690
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发表时间：
2018-09
期刊：
影响因子：
--
通讯作者：
Tang JL
中科院分区：
文献类型：
--
作者：
Zhang JL;Wang D;Liang YW;Zhong WY;Ming ZH;Tang DJ;Tang JL
蛋白质的合成对于大多数细菌至关重要。位于大都会的5'Utr然而，基因的重要性，在这里，甲基化的Xanthomonas属中的蛋氨酸生物合成途径包括许多重要的植物病原体。 XCC中的离子生物合成。甲基氨酸受限的培养基和植物印迹分析中的有缺陷表明，甲基氨基的表达取决于甲基氨酸水平，表明5'UTR，而不是歌剧的启动子，但与甲基的可用性相关的表达涉及甲基的表达。在甲基氨酸生物合成中。
The synthesis of methionine is critical for most bacteria. It is known that cellular methionine has a feedback effect on the expression of met genes involved in de novo methionine biosynthesis. Previous studies revealed that Gram-negative bacteria control met gene expression at the transcriptional level by regulator proteins, while most Gram-positive bacteria regulate met genes at post-transcriptional level by RNA regulators (riboregulators) located in the 5′UTR of met genes. However, despite its importance, the methionine biosynthesis pathway in the Gram-negative Xanthomonas genus that includes many important plant pathogens is completely uncharacterized. Here, we address this issue using the crucifer black rot pathogen Xanthomonas campestris pv. campestris (Xcc), a model bacterium in microbe–plant interaction studies. The work identified an operon (met) involved in de novo methionine biosynthesis in Xcc. Disruption of the operon resulted in defective growth in methionine-limited media and in planta. Western blot analysis revealed that the expression of the operon is dependent on methionine levels. Further molecular analyses demonstrated that the 5′UTR, but not the promoter of the operon, is involved in feedback regulation on operon expression in response to methionine availability, providing an example of a Gram-negative bacterium utilizing a 5′UTR region to control the expression of the genes involved in methionine biosynthesis.
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影响因子：
4.6
作者：
Li L;Li RF;Ming ZH;Lu GT;Tang JL
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DOI：
10.1016/j.bbagrm.2009.07.002
发表时间：
2009-09
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作者：
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DOI：
10.1073/pnas.83.22.8447
发表时间：
1986-11-01
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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