Green preparation of hierarchically structured hemostatic epoxy -amine sponge
Green preparation of hierarchically structured hemostatic epoxy -amine sponge
复制标题
分级结构环氧胺止血海绵的绿色制备
DOI：
10.1016/j.cej.2020.125445
复制
发表时间：
2020-10-01
影响因子：
15.1
通讯作者：
He, Wei
中科院分区：
文献类型：
--
作者：
Wan, Yafan;Han, Jianmei;He, Wei
具有高吸收性能的材料已被用于通过在伤口处形成物理屏障来控制出血。此外，研究表明，大孔隙率、阳离子化学基团和微粒形态是止血材料的有利属性。然而，到目前为止，还没有研究协同利用这些特性，并将它们整合到多功能材料的开发中，以增强出血止血的性能。在这里，我们介绍了一种新的止血海绵材料的制备方法，该方法简单、环保。该过程简单地包括环氧胺在水中的点击反应，以及聚合物溶液的温度诱导相分离现象。它能够将具有止血意义的多个关键特征汇聚到材料中。具体地说，相分离导致了环氧胺(EA)海绵的层次化结构，具有大孔道的高吸水率，而其微粒结构单元适合于包裹疏水客体。此外，EA海绵本身富含胺基，因此有可能表现出基于正电荷的促凝作用。该材料柔韧，表现出良好的血液相容性和细胞相容性。它还显示出比商业产品纱布和明胶海绵更好的止血性能，这得到了体外全血凝固研究以及活体小鼠尾巴截肢和大鼠肝损伤模型研究结果的支持。这种新的多功能材料已经显示出强有力的前景，可以保证进一步开发这种多方面的干预措施，以显著加强出血的止血管理。
Materials with highly absorptive property have been used to control bleeding by creating a physical barrier at the wound. Additionally, studies have suggested that macroporosity, cationic chemical groups, and microparticle morphology are advantageous attributes for hemostatic materials. Yet, no study to date has capitalized on these properties synergistically and integrates them into the development of multifunctional materials for enhanced performance in hemorrhage hemostasis. Here we present the preparation of such a new hemostatic sponge material using a facile and green method. The process simply involves epoxy-amine click reaction in water coupled with the phenomenon of temperature induced phase separation of polymer solution. It enables the convergence of multiple key features with hemostasis significance into the material. Specifically, phase separation results in the epoxy-amine (EA) sponge being hierarchically structured, with macroporosity for high water uptake while its microparticle structural units amenable for encapsulation of hydrophobic guests. Additionally, the EA sponge is inherently rich in amine groups, and thus can potentially exhibit a positive charge-based procoagulant effect. The material is pliable, and demonstrates excellent hemocompatibility and cytocompatibility. It also shows improved hemostatic performance over commercial products of gauze and gelatin sponge, supported by findings ofin vitrowhole blood clotting study, as well asin vivomouse-tail amputation and rat liver injury models. This new versatile material has shown strong promises to warrant further development of such a multifaceted intervention to significantly enhance hemostatic management of bleeding.