A BRIEF PHOTOCHEMICALLY INDUCED OXIDATIVE INSULT CAUSES IRREVERSIBLE LENS DAMAGE AND CATARACT .2. MECHANISM OF ACTION
A BRIEF PHOTOCHEMICALLY INDUCED OXIDATIVE INSULT CAUSES IRREVERSIBLE LENS DAMAGE AND CATARACT .2. MECHANISM OF ACTION
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DOI：
10.1016/s0014-4835(05)80063-6
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发表时间：
1995-05-01
影响因子：
3.4
通讯作者：
KLEIMAN, NJ
中科院分区：
文献类型：
--
作者：
SPECTOR, A;WANG, GM;KLEIMAN, NJ
使用光化学诱导的氧化应激和大鼠晶状体在器官培养中与4%O-2和4 μ M核黄素，已经发现，观察到的透镜参数的变化，在大多数情况下，是不可逆的。这使得阐明导致白内障的生物学变化的顺序成为可能。透镜细胞生物学中最早可检测的变化在上皮细胞氧化还原设定点和DNA水平（DNA完整性和H-3-胸苷掺入）中观察到，随后是膜转运减少和基因表达变化。经典白内障参数如水合作用、稳态非蛋白硫醇、甘油二酸脱氢酶-磷酸-脱氢酶活性和透明度的显著改变发生在较晚的时间。数据表明透镜破裂的明确模式，导致从上皮细胞水平开始的混浊，并导致随后的纤维细胞受累。
Using photochemically induced oxidative stress and rat lenses in organ culture with 4% O-2 and 4 mu M riboflavin, it has been found that the observed changes in lens parameters are, in most cases, irreversible. This has made possible the elucidation of the sequence of biological changes leading to cataract. The earliest detectable changes in lens cell biology are observed in the epithelial cell redox set point and at the DNA level in terms of DNA integrity and H-3-thymidine incorporation followed by decreased membrane transport and changes in gene expression. Significant modification in classical cataract parameters such as hydration, steady state non-protein thiol, glyceraldehyde-phosphate-dehydrogenase activity and transparency occur at later times. The data suggest a definitive pattern of lens breakdown resulting in opacity starting at the epithelial cell level and leading to subsequent fibre cell involvement.