Liquid chromatographic-photodiode array mass spectrometric analysis of dietary phytoestrogens from human urine and blood
Liquid chromatographic-photodiode array mass spectrometric analysis of dietary phytoestrogens from human urine and blood
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DOI：
10.1016/s1570-0232(02)00216-7
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发表时间：
2002-09-25
影响因子：
3
通讯作者：
Kolonel, LN
中科院分区：
文献类型：
--
作者：
Franke, AA;Custer, LJ;Kolonel, LN
膳食植物雌激素与预防慢性疾病有关。然而，尚不确定植物雌激素或与植物雌激素相关的食物是否能解释所观察到的效果。我们在这里报告了一种新的液相色谱光电二极管阵列质谱（LC-PDA-MS）测定法，用于测定人血浆或血清和尿液中最重要的膳食植物雌激素（金雀异黄酮、二氢金雀异黄酮、黄豆苷元、二氢黄豆苷元、黄豆黄素、O-去甲基安哥拉素、橙皮素、柚皮素、槲皮素、肠二醇、肠内酯）的纳摩尔量。发现该测定适用于通过测量尿槲皮素水平来评估洋葱干预研究中槲皮素的暴露量。该测定在临床和流行病学研究中的其他成功应用验证了所开发的方法，并证实了先前关于尿异黄酮排泄与乳腺癌风险之间负相关的结果。 (C) 2002 Elsevier Science B.V. 保留所有权利。
Dietary phytoestrogens have been implicated in the prevention of chronic diseases. However, it is uncertain whether the phytoestrogens or the foods associated with phytoestrogens account for the observed effects. We report here a new liquid chromatography photodiode array mass spectrometry (LC-PDA-MS) assay for the determination of nanomolar amounts of the most prominent dietary phytoestrogens (genistein, dihydrogenistein, daidzein, dihydrodaidzein, glycitein, O-desmethylangolensin, hesperetin, naringenin, quercetin, enterodiol, enterolactone) in human plasma or serum and urine. This assay was found to be suitable for the assessment of quercetin exposure in an onion intervention study by measuring urinary quercetin levels. Other successful applications of this assay in clinical and epidemiologic studies validated the developed method and confirmed previous results on the negative association between urinary isoflavone excretion and breast cancer risk. (C) 2002 Elsevier Science B.V. All rights reserved.