Thauera butanivorans sp nov., a C2-C9 alkane-oxidizing bacterium previously referred to as 'Pseudomonas butanovora'
Thauera butanivorans sp nov., a C2-C9 alkane-oxidizing bacterium previously referred to as 'Pseudomonas butanovora'
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DOI：
10.1099/ijs.0.000638-0
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发表时间：
2009-07-01
影响因子：
2.8
通讯作者：
Arp, Daniel J.
中科院分区：
文献类型：
--
作者：
Dubbels, Bradley L.;Sayavedra-Soto, Luis A.;Arp, Daniel J.
根据16 S rRNA基因序列分析，在进一步研究分类学特征的基础上，以前提出了“Pseudomonas butanovora”在Thauera属中的位置。本研究以生理特性及DNA-DNA重组资料，提出将“P. butanovora”转移至Thauera属。菌株（菌株Bu-B1211）的原始描述表明，它能够反硝化，但不能厌氧生长。“P. butanovora”能够厌氧呼吸和生长，在有机酸和醇的氧化过程中利用硝酸盐作为末端电子受体，但不利用芳烃或开链萜类化合物。总脂肪酸组成支持菌株Bu-B1211的Betaproteobacteria和类似的属Thauera的成员的分配。结合16 S rRNA基因系统发育证据、生理学和分类学特征以及DNA-DNA重新结合数据，支持将'Pseudomonasbutanovora' Bu-B1211作为一个新种，命名为Thauerabutanivoranssp. nov.。模式菌株为Bu-B1211（T）（=IAM 12574（T）=ATCC 43655（T）=DSM 2080（T））。
The placement of 'Pseudomonas butanovora' in the genus Thauera was proposed previously, based on 16S rRNA gene sequence analysis, upon further studies of taxonomical characteristics. In this study, physiological characteristics and DNA-DNA reassociation data are presented and the transfer of 'P. butanovora' to the genus Thauera is proposed. The original description of the strain (strain Bu-B1211) indicated that it was capable of denitrification but not anaerobic growth. 'P. butanovora' is capable of anaerobic respiration and growth, utilizing nitrate as a terminal electron acceptor during the oxidation of organic acids and alcohols, but not aromatic hydrocarbons or open-chain terpenoids. The total fatty acid composition supported the assignment of strain Bu-B1211 to the Betaproteobacteria and resembled that of members of the genus Thauera. The combination of 16S rRNA gene phylogenetic evidence, physiological and taxonomical characteristics and DNA-DNA reassociation data supported the placement of 'Pseudomonas butanovora' Bu-B1211 in the genus Thauera as representing a novel species, for which the name Thauera butanivorans sp. nov. is proposed. The type strain is Bu-B1211(T) (=IAM 12574(T)=ATCC 43655(T)=DSM 2080(T)).