Cloning of the human sodium iodide symporter
Cloning of the human sodium iodide symporter
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DOI：
10.1006/bbrc.1996.1358
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发表时间：
1996-09-13
影响因子：
3.1
通讯作者：
Jhiang, SM
中科院分区：
文献类型：
--
作者：
Smanik, PA;Liu, Q;Jhiang, SM
甲状腺的碘化物浓缩活性对于甲状腺激素的产生至关重要，也为放射性碘消融治疗甲状腺癌提供了机制。我们在这里报告了人类碘化钠同向转运体（hNIS）的核苷酸和氨基酸序列，它介导甲状腺中的碘化物吸收活性。 1929 个核苷酸的开放阅读框编码 643 个氨基酸的蛋白质，与大鼠 NIS (rNIS) 具有 84% 的同一性。 hNIS cDNA 的瞬时表达赋予非甲状腺细胞系 COS-7 对高氯酸盐敏感的碘化物摄取。在甲状腺乳头状癌组织中检测到不同水平的 hNIS 表达，但在任何失去碘摄取活性的甲状腺癌细胞系中均未检测到。 (C) 1996 学术出版社
The iodide concentrating activity of the thyroid gland is essential to the production of thyroid hormone and also provides a mechanism for the treatment of thyroid cancer by radioiodine ablation. We report here the nucleotide and amino acid sequence of the human sodium iodide symporter (hNIS), which mediates the iodide uptake activity in the thyroid gland. An open reading frame of 1929 nucleotides encodes a protein of 643 amino acids with 84% identity to the rat NIS (rNIS). Transient expression of the hNIS cDNA conferred perchlorate-sensitive iodide uptake to a nonthyroid cell line, COS-7. The expression of hNIS was detected at variable levels in papillary thyroid carcinoma tissues but not in any of the thyroid carcinoma cell lines that have lost the iodide uptake activity. (C) 1996 Academic Press, Inc.