Bioassay-guided isolation and identification of active compounds from Semen pharbitidis against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus).
Bioassay-guided isolation and identification of active compounds from Semen pharbitidis against Dactylogyrus intermedius (Monogenea) in goldfish (Carassius auratus).
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DOI：
10.1016/j.vetpar.2012.01.023
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发表时间：
2012-07
影响因子：
2.6
通讯作者：
B. Hao;Guanglu Liu;Xue-Gang Hu;Gaoxue Wang
中科院分区：
文献类型：
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作者：
B. Hao;Guanglu Liu;Xue-Gang Hu;Gaoxue Wang
本研究旨在分离牵牛子甲醇提取物对金鱼中间指环虫（Dactylogyrus intermedius）的驱虫活性成分。活性甲醇提取物在硅胶柱色谱法中进行生物测定引导的分级分离，通过将光谱数据（NMR和ESI-MS）与文献值进行比较，最终得到两种生物活性化合物：棕榈酸和pharnilatin A。根据体内驱虫试验，发现它们在5.3和1.4mgL−1的浓度（EC 50）下分别有50%的有效性。从S.此外，还进行了急性毒性试验，以评估其对宿主（金鱼）的安全性。48 h后，测定了金鱼的死亡率，其LC_（50）值分别为相应EC_（50）的2.45倍和5.29倍，表明pharnilatins A可能比棕榈酸具有更好的应用前景。本研究结果表明，pharnilatins A有可能成为防治指环虫的新型抗寄生虫药物。
The present study was undertaken to isolate the active compounds responsible for the anthelmintic activity of methanol extract of Semen pharbitidis against Dactylogyrus intermedius in goldfish (Carassius auratus). The active methanol extract was fractionated on silica gel column chromatography in a bioassay-guided fractionation, eventually yielding two bioactive compounds: palmitic acid and pharnilatin A by comparing spectral data (NMR and ESI-MS) with literature values. According to in vivo anthelmintic assays, they were found to be 50% effective at the concentrations (EC50) of 5.3 and 1.4mgL−1, respectively. The promising palmitic acid and pharnilatin A from S. pharbitidis were also subjected to acute toxicity tests for the evaluation of their safety to the host (goldfish). After 48h exposure, the mortalities of goldfish were recorded, and the established LC50values were 2.45- and 5.29-fold higher than the corresponding EC50, demonstrating that pharnilatins A may have better application potential than palmitic acid. The present results provide evidence that pharnilatins A might be potential source of new anti-parasitic drug for the control of Dactylogyrus.