Activation of nuclear factor-kappa B in the conjunctiva with the epithelial scraping of the mouse cornea and human epidemic keratoconjunctivitis
Activation of nuclear factor-kappa B in the conjunctiva with the epithelial scraping of the mouse cornea and human epidemic keratoconjunctivitis
复制标题
DOI：
10.1136/bjo.2003.024646
复制
发表时间：
2004-07-01
影响因子：
4.1
通讯作者：
Yoshida, K
中科院分区：
文献类型：
--
作者：
Kase, S;Aoki, K;Yoshida, K
目的：目的：检测核因子-κ B（NF-κ B）p65在C57 B16小鼠和1例流行性角膜结膜炎（EKC）患者结膜上皮中的表达。正常和EKC结膜上皮的样品，获得使用印模细胞学。结果：用抗NF-κ B p65抗体进行的免疫细胞化学显示，在未治疗的C57 B16小鼠中，p65定位于结膜上皮细胞的细胞质中。刮除角膜后6小时，p65蛋白在结膜上皮细胞核中表达。p65定位于人正常眼结膜上皮细胞的细胞质中。结论：NF-κ B在EKC患者结膜上皮细胞核中表达，提示NF-κ B在EKC患者结膜上皮细胞核中表达，在EKC患者结膜上皮细胞核中表达，提示NF-κ B在EKC患者结膜上皮细胞核中表达。
Aim: To examine the expression of p65, one of nuclear factor-kappa B (NF-kappaB), in the conjunctival epithelium of the C57Bl6 mouse and a patient with epidemic keratoconjunctivitis (EKC).Methods: Normal and epithelial scraped cornea obtained 6 hours after the injury were processed for paraffin section. Samples of a normal and an EKC conjunctival epithelium were obtained using impression cytology. Both samples were analysed by immunocytochemistry using anti-p65 antibody.Results: Immunocytochemistry with the anti-NF-kappaB p65 antibody revealed that p65 was localised in the cytoplasm of the conjunctival epithelium in the C57Bl6 mouse without the treatment. Six hours after the scraping of the cornea, p65 protein was expressed in the nuclei of the conjunctival epithelium. p65 was localised in the cytoplasm of the conjunctival epithelium in the human normal eye. p65 protein was expressed in the nuclei of the conjunctival epithelial cells in the EKC patient.Conclusion: These findings suggest that NF-kappaB was activated in the conjunctiva in the epithelial scraping of the mouse cornea and in human EKC.