Age-related alterations in the carbohydrate residue composition of the cell surface in the unexposed normal human epidermis
Age-related alterations in the carbohydrate residue composition of the cell surface in the unexposed normal human epidermis
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DOI：
10.1159/000083986
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发表时间：
2005-01-01
期刊：
影响因子：
3.5
通讯作者：
Tsambaos, D
中科院分区：
文献类型：
--
作者：
Georgiou, S;Pasmatzi, E;Tsambaos, D
背景：迄今为止，对正常人表皮的凝集素结合谱进行的极少数组织化学研究的结果大多是有争议的；因此，后者细胞表面的碳水化合物残基组成仍然存在争议，并且表皮凝集素结合谱的可能改变尚不清楚。目的：采用凝集素组织化学方法探讨年龄对未暴露的正常人表皮细胞表面碳水化合物残留组成的影响。方法：从防晒（未暴露）的臀部皮肤获取活检标本，分为 5 个年龄组，每组 18 名受试者，固定在缓冲福尔马林 (10%) 中并包埋在石蜡中。使用一组六种生物素化凝集素对 4-μm 切片进行组织化学处理。结果：在未暴露的正常人表皮中，各年龄组细胞表面β-D-半乳糖、D-半乳糖-β-(1,3 N-乙酰-D-半乳糖胺)、β(1,4D-N-乙酰-D-葡萄糖胺)和α-D-N-乙酰-D-半乳糖胺的浓度和分布几乎相同，而α-D-甘露糖、α-D-葡萄糖和α-L-岩藻糖显示出显着的年龄相关差异。结论：这些发现可能是由于单糖从细胞质到表皮细胞表面的合成和/或运输与年龄相关的下降。因此，相应的凝集素伴刀豆球蛋白 A 和 Ulex europaeus 凝集素-I 只能用于同龄个体正常和病理表皮细胞表面碳水化合物残留组成的比较组织化学研究，而蓖麻凝集素-I、花生凝集素、麦芽凝集素和 Dolichos biflorus 凝集素与碳水化合物的结合不受衰老影响，可用于比较组织化学研究。对任何年龄的人类受试者正常和病理表皮细胞表面碳水化合物残留组成的组织化学研究。版权所有 (C) 2005 S. Karger AG，巴塞尔。
Background: The results of the very few histochemical studies that have been performed so far on the lectin-binding profile of normal human epidermis are mostly controversial; thus, the carbohydrate residue composition of the cell surface in the latter still remains in dispute and the possible alterations in the epidermal lectin-binding profile are unknown. Objective: To investigate the influence of age on the carbohydrate residue composition of the cell surface in unexposed normal human epidermis by means of lectin histochemistry. Methods: Biopsy specimens obtained from the sun-protected (unexposed) buttock skin, divided into 5 age groups of 18 subjects each, were fixed in buffered formalin (10%) and embedded in paraffin. 4-mu m sections were processed for histochemistry using a panel of six biotinylated lectins. Results: In the unexposed normal human epidermis the concentration and distribution of cell surface beta-D-galactose, D-galactose-beta-(1,3 N-acetylo-D-galactosamine), beta(1,4D-N-acetylo-D-glucosamine) and alpha-D-N-acetylo-D-galactosamine were almost identical in all age groups, whereas those of alpha-D-mannose, alpha-D-glucose and alpha-L-fucose revealed significant age-related differences. Conclusion: These findings may be due to an age-related decline in synthesis and/or transport of monosaccharides from the cytoplasm to the surface of epidermal cells. Thus, the corresponding lectins concanavalin A and Ulex europaeus agglutinin-I can only be used in comparative histochemical studies of the carbohydrate residue composition of the cell surface in the normal and pathological epidermis of individuals of the same age, whereas Ricinus communis agglutinin-I, peanut agglutinin, wheat germ agglutinin and Dolichos biflorus agglutinin, whose binding to carbohydrates is not affected by aging can be used in histochemical studies of carbohydrate residue composition of the cell surface in the normal and pathological epidermis in human subjects of any age. Copyright (C) 2005 S. Karger AG, Basel.