Distance measurements via the morphogen gradient of Bicoid in Drosophila embryos.
Distance measurements via the morphogen gradient of Bicoid in Drosophila embryos.
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DOI：
10.1186/1471-213x-10-80
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发表时间：
2010-08-02
影响因子：
--
通讯作者：
Ma J
中科院分区：
文献类型：
--
作者：
He F;Wen Y;Cheung D;Deng J;Lu LJ;Jiao R;Ma J
果蝇胚胎沿前后轴（A-P）方向的模式是由Bicoid （Bcd）形态发生梯度指示的。尽管对这种形态发生进行了广泛的研究，但胚胎几何形状如何影响梯度形成和靶反应还没有实验研究。在本报告中，我们系统地比较了Bcd梯度分布及其在胚胎背侧和腹侧的靶表达模式。我们的结果支持一个假设，即适当的距离测量和Bcd梯度的编码位置信息沿着胚胎的周长。我们的研究结果还表明，胚胎的背侧和腹侧在Bcd与其靶驼背（Hb）之间具有基本相似的关系，这表明胚胎两侧的Hb表达特性可以直接追溯到Bcd梯度特性。我们的三维模拟研究表明，胚胎两侧的曲率差足以产生与实验观察一致的Bcd梯度特性。本报告中描述的发现提供了胚胎几何对Bcd梯度形成和靶反应的第一个定量实验评估。他们证明了胚胎的物理特征，比如它的形状，对图案的形成是不可或缺的。
Patterning along the anterior-posterior (A-P) axis in Drosophila embryos is instructed by the morphogen gradient of Bicoid (Bcd). Despite extensive studies of this morphogen, how embryo geometry may affect gradient formation and target responses has not been investigated experimentally. In this report, we systematically compare the Bcd gradient profiles and its target expression patterns on the dorsal and ventral sides of the embryo. Our results support a hypothesis that proper distance measurement and the encoded positional information of the Bcd gradient are along the perimeter of the embryo. Our results also reveal that the dorsal and ventral sides of the embryo have a fundamentally similar relationship between Bcd and its target Hunchback (Hb), suggesting that Hb expression properties on the two sides of the embryo can be directly traced to Bcd gradient properties. Our 3-D simulation studies show that a curvature difference between the two sides of an embryo is sufficient to generate Bcd gradient properties that are consistent with experimental observations. The findings described in this report provide a first quantitative, experimental evaluation of embryo geometry on Bcd gradient formation and target responses. They demonstrate that the physical features of an embryo, such as its shape, are integral to how pattern is formed.