Novel cynomolgus macaque MHC-DPB1 polymorphisms in three South-East Asian populations
Novel cynomolgus macaque MHC-DPB1 polymorphisms in three South-East Asian populations
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DOI：
10.1111/j.1399-0039.2006.00577.x
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发表时间：
2006-04-01
期刊：
影响因子：
--
通讯作者：
Inoko, H
中科院分区：
文献类型：
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作者：
Sano, K;Shiina, T;Inoko, H
食蟹猴（Macaca fascicularis，Mafa），又名食蟹猴或长尾猕猴，生活在东南亚的广大地区。这些非人灵长类动物已成为重要的动物模型，在传染病和慢性疾病和移植研究，需要更广泛的表征其主要组织相容性复合体多态性区域。与更常见的研究恒河猴DPB 1基因座相比，Mafa-DPB 1基因座的多态性变异或多样性的当前信息在很大程度上是有限的。本文为了更好地阐明三个东南亚群体中Mafa-DPB 1基因座多态性的程度和类型以及遗传差异，并探讨猕猴和人类之间的等位基因差异如何影响各自的免疫反应，我们通过DNA测序方法鉴定了Mafa-DPB 1基因座外显子2内的40个等位基因。使用217名个体。我们还进行了进化和人口分析，使用这些序列，以揭示一些人口特异性等位基因和跨物种之间的等位基因的食蟹猴和恒河猴的保守性。在40个新的等位基因中，有8个属于一个新发现的谱系组，以前在恒河猴物种中没有发现。这些等位基因信息将有助于医学研究人员在疾病和免疫学研究中使用食蟹猴。
Cynomolgus macaques (Macaca fascicularis, Mafa), alias the crab-eating monkeys or long-tailed macaques, live across a vast range of South-East Asia. These non-human primates have emerged as important animal models in infectious and chronic diseases and transplantation studies, necessitating a more extensive characterization of their major histocompatibility complex polymorphic regions. The current information on the polymorphic variation or diversity of the Mafa-DPB1 locus is largely limited in comparison with the more commonly studied rhesus macaque DPB1 locus. In this article, to better elucidate the degree and types of polymorphisms and genetic differences of Mafa-DPB1 locus among three South-East Asian populations and to investigate how the allele differences between macaques and humans might affect their respective immune responses, we identified 40 alleles within exon 2 of the Mafa-DPB1 locus by DNA sequencing using 217 individuals. We also performed evolutionary and population analyses using these sequences to reveal some population-specific alleles and trans-species allelic conservation between the cynomolgus macaques and the rhesus macaques. Of the 40 new alleles, eight belong to a newly identified lineage group not previously found in the rhesus macaque species. This allele information will be useful for medical researchers using the cynomolgus macaques in disease and immunological studies.