Lethal activity of miconazole in Neisseria gonorrhoeae grown in pure and mixed cultures
Lethal activity of miconazole in Neisseria gonorrhoeae grown in pure and mixed cultures
复制标题
咪康唑对纯培养和混合培养的淋病奈瑟菌的致死活性
DOI：
--
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
M. Jacques
中科院分区：
文献类型：
--
作者：
L. Mathieu;M. Jacques
低浓度(如2×10−6M)咪唑类抗真菌药咪康唑对革兰氏阴性兼性需氧菌淋病奈瑟菌有致死作用，无论是单独培养还是与白色念珠菌混合培养。咪康唑对奈瑟氏奈瑟菌细胞的电子显微镜观察显示，细胞外壁存在气泡，细胞外壁与细胞膜之间有分离区。细胞裂解产物的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)没有显示任何一个菌株的细胞在处理和未处理的细胞之间的主要外膜蛋白的差异。咪唑类化合物常用于治疗念珠菌病。我们的体外结果表明，低浓度的咪康唑对淋病奈瑟菌有杀菌作用，淋球菌是一种可以在人体内经常发现念珠菌的部位定居的细菌。
Low concentrations (e.g. 2 × 10−6 M) of an imidazole derivative anti-fungal agent, miconazole, were lethal for the Gram-negative, facultative aerobic pathogen Neisseria gonorrhoeae grown either alone or in mixed culture with the yeast Candida albicans. Electron microscopic observation of Neisseria cells exposed to miconazole showed the presence of blebs in the outer wall and areas of separation between the wall and the cytoplasmic membrane. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) of cell lysates did not reveal differences in major outer membrane proteins between the treated and the untreated cells of any one strain. Imidazole derivatives are frequently used in the treatment of candidiasis. Our in vitro results show that low concentrations of one of them, miconazole, can be bactericidal for N. gonorrhoeae, a bacterium that can colonise sites of the human body where Candida is often found.