Wheat NAC transcription factor NAC5-1 is a positive regulator of senescence
Wheat NAC transcription factor NAC5-1 is a positive regulator of senescence
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小麦 NAC 转录因子 NAC5-1 是衰老的正调节因子
DOI：
10.1101/2024.02.02.578592
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Evans C
中科院分区：
文献类型：
--
作者：
Evans C
小麦（Triticum aestivumL.）谷物是全球饮食中热量和蛋白质的重要来源，但谷物产量和蛋白质含量之间存在权衡。叶片衰老的时间可以介导这种权衡，因为它与光合作用和氮从叶片到籽粒的再动员的下降有关。NAC转录因子在调节衰老时间中起关键作用。在水稻中，OsNAC 5表达与蛋白质含量增加相关，并且在衰老叶片中上调，但小麦直系同源物在衰老中的作用尚未表征。我们验证了NAC 5 - 1是OsNAC 5的直向同源物，并且在小麦衰老的旗叶中表达。为了表征NAC 5 - 1，我们将TILLING突变群体中的NAC 5-A1和NAC 5-B1中的错义突变与小麦中的过表达NAC 5-A1相结合。NAC 5 - 1的突变与旗叶衰老的延迟发生相关，而NAC 5-A1的过表达与叶片衰老的略微提前发生相关。进行DAP-seq以定位NAC 5 - 1的转录因子结合位点。对DAP-seq的分析以及与其他研究的比较确定了NAC 5 - 1的推定下游靶基因，这些基因可能与衰老相关。这项工作表明NAC 5 - 1是小麦叶片衰老的正向转录调控因子。还需要进一步的研究来测试NAC 5 - 1在田间试验中对产量和蛋白质含量的影响，以评估利用这种衰老调节剂开发高产小麦同时保持籽粒蛋白质含量的潜力。
Wheat (Triticum aestivumL.) is an important source of both calories and protein in global diets, but there is a trade‐off between grain yield and protein content. The timing of leaf senescence could mediate this trade‐off as it is associated with both declines in photosynthesis and nitrogen remobilization from leaves to grain. NAC transcription factors play key roles in regulating senescence timing. In rice,OsNAC5expression is correlated with increased protein content and upregulated in senescing leaves, but the role of the wheat ortholog in senescence had not been characterized. We verified thatNAC5‐1is the ortholog ofOsNAC5and that it is expressed in senescing flag leaves in wheat. To characterizeNAC5‐1, we combined missense mutations inNAC5‐A1andNAC5‐B1from a TILLING mutant population and overexpressedNAC5‐A1in wheat. Mutation inNAC5‐1was associated with delayed onset of flag leaf senescence, while overexpression ofNAC5‐A1was associated with slightly earlier onset of leaf senescence. DAP‐seq was performed to locate transcription factor binding sites ofNAC5‐1. Analysis of DAP‐seq and comparison with other studies identified putative downstream target genes ofNAC5‐1which could be associated with senescence. This work showed thatNAC5‐1is a positive transcriptional regulator of leaf senescence in wheat. Further research is needed to test the effect ofNAC5‐1on yield and protein content in field trials, to assess the potential to exploit this senescence regulator to develop high‐yielding wheat while maintaining grain protein content.
影响因子：
3
作者：
Daniel E;Onwukwe GU;Wierenga RK;Quaggin SE;Vainio SJ;Krause M
通讯作者：
Krause M