An in vitro study of the potential of carbon nanotubes and nanofibres to induce inflammatory mediators and frustrated phagocytosis
An in vitro study of the potential of carbon nanotubes and nanofibres to induce inflammatory mediators and frustrated phagocytosis
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DOI：
10.1016/j.carbon.2007.05.011
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发表时间：
2007-08-01
期刊：
影响因子：
10.9
通讯作者：
Stone, V.
中科院分区：
文献类型：
--
作者：
Brown, D. M.;Kinloch, I. A.;Stone, V.
尽管碳纳米纤维材料在工业中的使用越来越多，但迄今为止，关于工人吸入碳纳米纤维材料对健康的潜在影响和危害的信息很少。这项研究检验了一系列纳米纤维和纳米管刺激单核细胞释放促炎细胞因子 TNF-α 和活性氧 (ROS) 的能力的体外效应。还评估了纳米材料对细胞的毒性作用以及暴露后细胞的吞噬能力。我们的研究表明，细胞反应随纤维形态和聚集状态而变化。与高度弯曲和缠结的材料相比，长、直、分散良好的纳米丝在单核细胞中产生明显更多的 TNF-α 和 ROS。我们还证明，暴露于本研究中使用的所有纳米管后，单核细胞的吞噬能力降低。用纳米管处理后的细胞显微镜检查显示“吞噬作用受阻”。吞噬作用受阻表明巨噬细胞从肺部清除纳米管的能力可能受到损害。没有证据表明在使用的任何剂量或时间点有毒性作用。这些考虑因素可能会对接触这些纳米材料的工人产生重要影响。 (c) 2007 Elsevier Ltd. 保留所有权利。
There has been little information to date based on the potential health effects and hazards associated with the inhalation of carbon nanofibrous materials by workers despite their growing use in industry. This study examines the in vitro effects of a range of nanofibres and nanotubes for their ability to stimulate the release of the pro-inflammatory cytokine TNF-alpha and reactive oxygen species (ROS) from monocytic cells. Also assessed were the toxic effect of the nanomaterials on the cells and the phagocytic ability of the cells after exposure. Our studies showed that the cellular response varied with fibre morphology and state of aggregation; long, straight, well-dispersed nanofilaments produced significantly more TNF-alpha and ROS in monocytic cells compared with highly curved and entangled materials. We also demonstrated that monocytic cell phagocytic ability was reduced after exposure to all of the nanotubes used in this study. Microscopic examination of the cells after treatment with the nanotubes showed 'frustrated phagocytosis', The frustrated phagocytosis suggests that clearance of nanotubes from the lungs by macrophages may be impaired. There was no evidence of a toxic effect at any of the doses or time points used. These considerations may have important consequences for workers exposed to these nanomaterials. (c) 2007 Elsevier Ltd. All rights reserved.