The Elav-like proteins bind to AU-rich elements and to the poly(A) tail of mRNA
The Elav-like proteins bind to AU-rich elements and to the poly(A) tail of mRNA
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DOI：
10.1093/nar/25.18.3564
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发表时间：
1997-09-15
影响因子：
14.9
通讯作者：
Furneaux, H
中科院分区：
文献类型：
--
作者：
Ma, WJ;Chung, S;Furneaux, H
Elav样蛋白是细胞分化所必需的特异的信使核糖核酸结合蛋白。它们含有三个特征的RNP2/RNP1型RNA结合基序。先前我们已经证明，第一和第二个RNA结合域与mRNA的3‘-UTR区中的富含AU的元素结合。在这篇文章中，我们证明了类Elav蛋白具有聚(A)结合活性。该活性不同于先前描述的聚(A)结合活性。类Elav蛋白与长链聚(A)尾巴特异结合。我们已经证明了第三个RNA结合域包含这种聚(A)结合活性。使用聚(A)琼脂糖珠在“三明治”实验中，我们已经证明了Elav样蛋白可以同时与富含AU的元件和PolyA尾巴结合。
The Elav-like proteins are specific mRNA binding proteins which are required for cellular differentiation. They contain three characteristic RNP2/RNP1-type RNA binding motifs. Previously we have shown that the first and second RNA binding domains bind to AU-rich elements in the 3'-UTR of mRNA. In this paper we show that the Elav-like proteins exhibit poly(A) binding activity. This activity is distinct from poly(A) binding activities that have been previously described. The Elav-like proteins specifically bind to long chain poly(A) tails. We have shown that the third RNA binding domain encompasses this poly(A) binding activity. Using poly(A)Sepharose beads in a 'sandwich' assay we have shown that the Elav-like proteins can bind simultaneously to the AU-rich element and to the poly(A) tail.