Upregulation of Insulin-Like Growth Factor Binding Protein 3 in Astrocytes of Transgenic Mice That Express Borna Disease Virus Phosphoprotein
Upregulation of Insulin-Like Growth Factor Binding Protein 3 in Astrocytes of Transgenic Mice That Express Borna Disease Virus Phosphoprotein
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DOI：
10.1128/jvi.01817-10
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发表时间：
2011-05-01
影响因子：
5.4
通讯作者：
Tomonaga, Keizo
中科院分区：
文献类型：
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作者：
Honda, Tomoyuki;Fujino, Kan;Tomonaga, Keizo
在先前的研究中，我们证明了在星形胶质细胞中表达博尔纳病病毒（BDV）磷蛋白（P）的转基因小鼠显示出与BDV感染动物相似的显著神经行为异常。为了了解P在星形胶质细胞中的表达诱导的分子干扰，我们进行了微阵列分析与培养的星形胶质细胞瞬时表达P.我们发现，胰岛素样生长因子结合蛋白3 mRNA的表达增加不仅在P-表达培养细胞，但也在星形胶质细胞从小脑的P转基因小鼠（P-Tg）。此外，我们证明，胰岛素样生长因子信号被干扰的P-Tg小脑，一个因素，可能参与了在大脑中的浦肯野细胞神经元的脆弱性增加。
In a previous study, we demonstrated that transgenic mice that express Borna disease virus (BDV) phosphoprotein (P) in astrocytes show striking neurobehavioral abnormalities resembling those in BDV-infected animals. To understand the molecular disturbances induced by the expression of P in astrocytes, we performed microarray analysis with cultured astroglial cells transiently expressing P. We showed that expression of insulin-like growth factor binding protein 3 mRNA increases not only in P-expressing cultured cells but also in astrocytes from the cerebella of P transgenic mice (P-Tg). Furthermore, we demonstrated that insulin-like growth factor signaling is disturbed in the P-Tg cerebellum, a factor that might be involved in the increased vulnerability of Purkinje cell neurons in the brain.