Immunohistochemical Identification of Myoepithelial, Epithelial, and Connective Tissue Cells in Canine Mammary Tumors
Immunohistochemical Identification of Myoepithelial, Epithelial, and Connective Tissue Cells in Canine Mammary Tumors
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犬乳腺肿瘤中肌上皮、上皮和结缔组织细胞的免疫组织化学鉴定
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
F. Coignoul
中科院分区：
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作者：
E. Destexhe;L. Lespagnard;Marianne Degeyter;R. Heymann;F. Coignoul
58个福尔马林固定石蜡包埋的犬乳腺肿瘤，19个恶性和39个良性，在这项研究中使用。肿瘤取自布鲁塞尔私人兽医诊所或列日大学兽医学院外科部门提交进行病变手术切除的犬。使用针对角蛋白8-18和19、波形蛋白、结蛋白和α-肌动蛋白的单克隆抗体以及针对高分子量角蛋白和S-100蛋白的多克隆抗体进行免疫组织化学评价。主要的细胞类型，上皮，肌上皮，结缔组织，被确定，肌上皮细胞代表了大多数肿瘤的主要组成部分，良性和恶性。肌上皮细胞有五种类型：静止和增殖基底上细胞，梭形和星形间质细胞，和软骨。对角蛋白19、波形蛋白、α-肌动蛋白和S-100蛋白的反应性表明从静息细胞向软骨的进行性转化。上皮细胞的反应性仅限于角蛋白，只有角化细胞的多克隆角蛋白呈阳性。肌成纤维细胞波形蛋白和α-肌动蛋白均呈阳性，结缔组织细胞波形蛋白呈阳性。肌上皮细胞似乎是癌的主要组成部分，证明重新评估和简化组织形态学分类，与“多形性癌”组包括所有的癌，除了鳞状细胞癌，粘液癌，粉刺癌。除了常规的苏木精和伊红组织病理学评价外，建议进行免疫组织化学评价以精确分类犬乳腺肿瘤。
Fifty-eight formalin-fixed paraffin-embedded canine mammary tumors, 19 malignant and 39 benign, were used in this study. Tumors were obtained from dogs submitted for surgical resection of lesions at private veterinary practices in Brussels or from the surgery unit of the Faculty of Veterinary Medicine, University of Liège. Immunohistochemical evaluation was performed, using monoclonal antibodies directed against keratins 8–18 and 19, vimentin, desmin, and α-actin and polyclonal antibodies directed against high-molecular-weight keratins and S-100 protein. The main cell types, epithelial, myoepithelial, and connective, were identified, and myoepithelial cells represented the major component of most tumors, both benign and malignant. Myoepithelial cells had five patterns: resting and proliferative suprabasal cells, spindle and star-shaped interstitial cells, and cartilage. Reactivity to keratin 19, vimentin, α-actin, and S-100 protein suggested a progressive transformation from resting cells to cartilage. Epithelial cell reactivities were limited to keratins; only keratinized cells were positive for polyclonal keratins. Myofibroblasts were positive for both vimentin and α-actin, and connective tissue cells were positive for vimentin. Myoepithelial cells appeared to be the major component of carcinomas, justifying reevaluation and simplification of histomorphologic classifications, with a “pleomorphic carcinoma” group including all carcinomas except squamous, mucinous, and comedo carcinomas. Immunohistochemical evaluation, in addition to routine hematoxylin and eosin histopathologic evaluation is recommended for precise classification of canine mammary tumors.