Amplicons on human chromosome 11q are located in the early/late-switch regions of replication timing
Amplicons on human chromosome 11q are located in the early/late-switch regions of replication timing
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DOI：
10.1016/j.ygeno.2004.08.001
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发表时间：
2004-11-01
期刊：
影响因子：
4.4
通讯作者：
Sugimura, H
中科院分区：
文献类型：
--
作者：
Watanabe, Y;Ikemura, T;Sugimura, H
扩增子经常在人类肿瘤基因组中发现，但其产生的机制仍然知之甚少。我们先前测量了沿着人类染色体I I q和21 q全长的基因的复制时间，发现许多“疾病相关”基因位于时间过渡区。在这项研究中，进一步审查更新的复制定时人类染色体I1 q的地图显示，两个扩增子的人类染色体带I1 q13和I1 q22位于早期/晚期开关区域的复制定时在两个人类细胞系（THP-1和Jurkat）。此外，对人类和小鼠基因组中的同线性的检查显示，两种基因组中的同线性断裂主要发生在我们已经确定的复制时间的早期/晚期转换区域。总之，我们发现，复制时间的早期/晚期转换区域与基因组的“不稳定”区域相吻合。(C)2004年爱思唯尔公司All rights reserved.
Amplicons are frequently found in human tumor genomes, but the mechanism of their generation is still poorly understood. We previously measured the replication timing of the genes along the entire length of human chromosomes I I q and 21 q and found that many "disease-related" genes are located in timing-transition regions. In this study, further scrutiny of the updated replication-timing map of human chromosome I 1q revealed that both amplicons on human chromosomal bands I 1q13 and I I q22 are located in the early/late-switch regions of replication timing in two human cell lines (THP-1 and Jurkat). Moreover, examination of synteny in the human and mouse genomes revealed that synteny breakage in both genomes occurred primarily at the early/late-switch regions of replication timing that we had identified. In conclusion, we found that the early/late-switch regions of replication timing coincided with "unstable" regions of the genome. (C) 2004 Elsevier Inc. All rights reserved.