Loss of Periostin Results in Impaired Regeneration and Pancreatic Atrophy after Cerulein-Induced Pancreatitis
Loss of Periostin Results in Impaired Regeneration and Pancreatic Atrophy after Cerulein-Induced Pancreatitis
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DOI：
10.1016/j.ajpath.2015.09.022
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发表时间：
2016-01-01
影响因子：
6
通讯作者：
Erkan, Mert
中科院分区：
文献类型：
--
作者：
Hausmann, Simone;Regel, Ivonne;Erkan, Mert
细胞外基质分子Periostin(POSTN)由活化的胰星状细胞分泌，由POSTN编码，在慢性胰腺炎和胰腺癌中具有重要作用。然而，POSTN在急性胰腺炎和随后的再生过程中的作用到目前为止还没有得到解决。我们分析了POSTN在重症急性胰腺炎诱导后胰腺外分泌再生中的作用。POSTN缺陷小鼠和野生型对照动物接受重复注射蓝蛋白，并对胰腺组织进行详细的组织学分析。尽管在急性炎症阶段胰腺炎的严重程度没有差别，但在Postn缺乏的小鼠中，外分泌胰腺的恢复受到了严重的损害。Postn表达的缺失伴随着强烈的胰腺萎缩和腺泡向脂肪细胞的分化，这也反映在基因表达模式上。我们的数据表明，POSTN是重症急性胰腺炎后适当的外分泌谱系特异性再生的关键因素。
The extracellular matrix molecule periostin (POSTN, encoded by POSTN), which is secreted by activated pancreatic stellate cells, has important functions in chronic pancreatitis and pancreatic cancer. However, the role of POSTN in acute pancreatitis and subsequent regeneration processes has not been addressed so far. We analyzed the function of POSTN in pancreatic exocrine regeneration after the induction of a severe acute pancreatitis. Postn-deficient mice and wild-type control animals received repetitive cerulein injections, and a detailed histologic analysis of pancreatic tissues was performed. Although there was no difference in pancreatitis severity in the acute inflammatory phase, the recovery of the exocrine pancreas was massively impaired in Postn-deficient mice. Loss of Postn expression was accompanied by strong pancreatic atrophy and acinar-to-adipocyte differentiation, which was also reflected in gene expression patterns. Our data suggest that POSTN is a crucial factor for proper exocrine lineage-specific regeneration after severe acute pancreatitis.