Cloning and overexpression of ribosomal protein L39 gene from deltamethrin-resistant Culex pipiens pallens
Cloning and overexpression of ribosomal protein L39 gene from deltamethrin-resistant Culex pipiens pallens
复制标题
耐溴氰菊酯淡色库蚊核糖体蛋白L39基因的克隆和过表达。
DOI：
10.1016/j.exppara.2006.09.020
复制
发表时间：
2007-04-01
影响因子：
2.1
通讯作者：
Zhu, Changliang
中科院分区：
文献类型：
--
作者：
Tan, Wenbin;Sun, Lixin;Zhu, Changliang
从淡色库蚊溴氰菊酯抗性品系（DR 1株）中克隆了核糖体蛋白L39的全序列。定量RT-PCR分析表明，在4龄幼虫中，DR 1品系的RPL 39转录水平是敏感品系的23.4倍。还发现RPL 39表达在DR 1菌株的整个生命周期中始终较高。在RPL 39转染的蚊子C6/36细胞中通过Western印迹检测到预测大小为17 kDa的蛋白。通过甲基氚化胸苷（H-3-TdR）掺入测定，与质粒载体转染细胞相比，这些RPL 39转染细胞也显示出增强的溴氰菊酯抗性。这些结果表明，RPL 39在DR 1菌株中以较高水平表达，并且可能赋予Cx一定的杀虫剂抗性。pipiens pallens (c)2006年爱思唯尔公司All rights reserved.
The complete sequence of ribosomal protein L39 has been cloned from deltamethrin-resistant strain of Cidex pipiens pallens (DR1 strain). Quantitative RT-PCR analysis indicated that the RPL39 transcription level was 23.4 times higher in DR1 strain than in susceptible strain at 4th instar larvae. The RPL39 expression was also found to be consistently higher throughout the life cycle of DR1 strain. A protein of predicted size 17 kDa has been detected by Western blotting in RPL39-transfected mosquito C6/36 cells. These RPL39-transfected cells also showed enhanced deltamethrin resistance compared to plasmid vector-transfected cells as determined by methyl tritiated thymidine (H-3-TdR) incorporation. These results indicate that RPL39 is expressed at higher levels in DR1 strain, and may confer some insecticide resistance in Cx. pipiens pallens. (c) 2006 Elsevier Inc. All rights reserved.