Partial amino acid sequences of the heavy and light chains of botulinum neurotoxin type E.
Partial amino acid sequences of the heavy and light chains of botulinum neurotoxin type E.
复制标题
E型肉毒杆菌神经毒素重链和轻链的部分氨基酸序列。
DOI：
10.1016/s0006-291x(85)80009-7
复制
发表时间：
1985
影响因子：
3.1
通讯作者：
DasGupta,BR
中科院分区：
文献类型：
--
作者：
Sathyamoorthy,V;DasGupta,BR
双链型E型肉毒神经毒素是由胰蛋白酶切割单链蛋白质而产生的，由重链和轻链组成。测定了这两条链的前13和20个N-末端残基的序列。此外，本文提供了以下证据：（i）切口（双链）的轻链衍生自亲本单链神经毒素的N-末端三分之一，和（ii）导致单链神经毒素活化的分子事件不涉及在单链蛋白的N-末端处或非常接近N-末端的胰蛋白酶切割。E型肉毒杆菌和破伤风神经毒素的轻链的部分氨基酸序列显示两种梭菌神经毒素之间的显著相似性。
The dichain type E botulinum neurotoxin, a product of nicking the single chain protein by trypsin, is composed of a heavy and light chains. Sequence of the first 13 and 20 N-terminal residues of these two chains were determined. Also, proof is provided here that (i) the light chain of the nicked (dichain) is derived from the N-terminal one-third of the parent single chain neurotoxin, and (ii) molecular events leading to the activation, of the single chain neurotoxin cannot involve tryptic cleavage at or very close to the N-terminal of the single chain protein. The partial amino acid sequence of the light chain of botulinum type E and tetanus neurotoxins show significant similarity between the two clostridial neurotoxins.