Lipopolysaccharide induced expression of pentraxin 3 in human neutrophils and monocyte-derived macrophages
Lipopolysaccharide induced expression of pentraxin 3 in human neutrophils and monocyte-derived macrophages
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DOI：
10.1016/j.cellimm.2007.09.003
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发表时间：
2007-08-01
影响因子：
4.3
通讯作者：
Naito, Makoto
中科院分区：
文献类型：
--
作者：
Imamura, Masaru;Kawasaki, Takashi;Naito, Makoto
正五聚蛋白3（PTX 3）是长正五聚蛋白的原型蛋白。PTX 3由多种细胞产生，例如单核细胞/巨噬细胞（M phi s）响应于脂多糖（LPS）和促炎信号。免疫印迹、免疫组化染色。逆转录-聚合酶链反应（RT-PCR）、实时定量PCR和酶联免疫吸附试验（ELISA）检测人单核细胞源性M phi s和中性粒细胞中PTX 3的表达。在GM-CSF诱导的单核细胞衍生的M phi（GM-M phi）和M-CSF诱导的单核细胞衍生的M phi（M-M phi）的细胞质中观察到PTX 3表达。LPS刺激后24 μ l，两种M phi中的PTX 3水平均上调。此外，我们证实了PTX 3在新鲜分离的中性粒细胞中的表达，并且PTX 3水平在LPS刺激后在6和24小时明显上调。这些结果表明，PTX 3的表达，不仅在M phi s，而且在中性粒细胞，可能反映了PTX 3在炎症中的作用。我们认为，PTX 3可以作为一种诊断工具，以评估炎症在外周网站。(C)2007爱思唯尔公司All rights reserved.
Pentraxin 3 (PTX3) is a prototype protein of long pentraxin. PTX3 is produced by various cells, such as monocytes/macrophages (M phi s) in response to lipopolysaccharide (LPS) and proinflammatory signals. We performed immunoblotting, immunohistochemical staininig. reverse transcriptase-polymerase chain reaction (RT-PCR), quantitative real-time PCR and enzyme-linked immunosorbent assay (ELISA) of PTX3 in human monocyte-derived M phi s and neutrophils. PTX3 expression was observed in the cytoplasm of both GM-CSF induced monocyte-derived M phi (GM-M phi) and M-CSF induced monocyte-derived M phi (M-M phi). PTX3 level in both M phi s was up-regulated at 24 It after LPS stimulation. Moreover, we confirmed PTX3 expression in freshly isolated neutrophils, and PTX3 level was distinctly up-regulated at 6 and 24 It after LPS stimulation. These findings suggested that PTX3 expression, not only in M phi s, but also in neutrophils, may reflect the role of PTX3 in inflammation. We believe that PTX3 can contribute as a diagnostic tool to evaluate inflammation at peripheral sites. (C) 2007 Elsevier Inc. All rights reserved.