THE URE2 GENE-PRODUCT OF SACCHAROMYCES-CEREVISIAE PLAYS AN IMPORTANT ROLE IN THE CELLULAR-RESPONSE TO THE NITROGEN-SOURCE AND HAS HOMOLOGY TO GLUTATHIONE S-TRANSFERASES
THE URE2 GENE-PRODUCT OF SACCHAROMYCES-CEREVISIAE PLAYS AN IMPORTANT ROLE IN THE CELLULAR-RESPONSE TO THE NITROGEN-SOURCE AND HAS HOMOLOGY TO GLUTATHIONE S-TRANSFERASES
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DOI：
10.1128/mcb.11.2.822
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发表时间：
1991-02-01
影响因子：
5.3
通讯作者：
MAGASANIK, B
中科院分区：
文献类型：
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作者：
COSCHIGANO, PW;MAGASANIK, B
本研究克隆了酿酒酵母URE 2基因并进行了序列测定。 它编码354个氨基酸的预测多肽，分子量为40，226。 前63个氨基酸的缺失对蛋白质的功能没有任何影响。 URE 2和GLN 3基因的破坏等位基因的研究表明，这两个基因调节GLN 1和GDH 2，谷氨酰胺合成酶和NAD-连接的谷氨酸脱氢酶的结构基因，分别在转录水平，但调控基因的表达似乎不受调节。 在以谷氨酸为氮源生长的细胞中加入谷氨酰胺后，需要活性URE 2基因产物来灭活谷氨酰胺合成酶。 预测的URE 2基因产物与谷胱甘肽S-转移酶具有同源性。 该基因已被定位到染色体XIV，5.9地图单位从petX和3.4地图单位从kex 2。
The URE2 gene of Saccharomyces cerevisiae has been cloned and sequenced. It encodes a predicted polypeptide of 354 amino acids with a molecular weight of 40,226. Deletion of the first 63 amino acids does not have any effect on the function of the protein. Studies with disruption alleles of the URE2 and GLN3 genes showed that both genes regulate GLN1 and GDH2, the structural genes for glutamine synthetase and NAD-linked glutamate dehydrogenase, respectively, at the transcriptional level, but expression of the regulatory genes does not appear to be regulated. Active URE2 gene product was required for the inactivation of glutamine synthetase upon addition of glutamine to cells growing with glutamate as the source of nitrogen. The predicted URE2 gene product has homology to glutathione S-transferases. The gene has been mapped to chromosome XIV, 5.9 maps units from petX and 3.4 map units from kex2.