Engineering of epidermis skin grafts using electrospun nanofibrous gelatin/ polycaprolactone membranes.
Engineering of epidermis skin grafts using electrospun nanofibrous gelatin/ polycaprolactone membranes.
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DOI：
10.2147/ijn.s42384
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发表时间：
2013
影响因子：
8
通讯作者：
Zhang WJ
中科院分区：
文献类型：
--
作者：
Duan H;Feng B;Guo X;Wang J;Zhao L;Zhou G;Liu W;Cao Y;Zhang WJ
皮肤工程为治疗各种皮肤缺损提供了新的策略。特别是，静电纺丝纳米纤维膜已被用作表皮工程的载体。本研究的目的是探讨一种改性明胶/聚己内酯（GT/PCL）电纺膜用于表皮工程的可行性。通过在膜上接种HaCaT细胞（人角质形成细胞系）来评价膜的生物相容性，并在培养后测定有细胞和无细胞的膜的机械性能。细胞增殖实验显示HaCaT细胞在膜上粘附和增殖良好，表明膜具有良好的生物相容性。机械测试表明，膜的强度足以在移植过程中处理。进一步的体内移植研究表明，用GT/PCL膜工程化的表皮能够修复裸鼠的皮肤缺损。这些结果表明，GT/PCL静电纺丝膜可以作为皮肤工程的合适支架。
Skin engineering provides a new strategy for treating a wide variety of skin defects. In particular, electrospun nanofibrous membranes have been used as carriers for epidermis engineering. The aim of this study was to investigate the feasibility of a modified gelatin and polycaprolactone (GT/PCL) electrospun membrane for epidermis engineering. The biocompatibility of the membranes was evaluated by seeding HaCaT cells (human keratinocyte cell line) on the membrane and the mechanical properties of the membranes were determined with and without cells after culture. A cell proliferation assay showing that HaCaT cells attached and proliferated well on the membranes demonstrated that the membranes possess good biocompatibility. Mechanical tests showed that the membranes are strong enough to be handled during transplantation. Further in vivo transplantation studies revealed that epidermises engineered with GT/PCL membranes were able to repair skin defects in the nude mouse. These results demonstrate that GT/PCL electrospun membranes could be suitable scaffolds for skin engineering.