c-Fos expression as endogenous marker of lumbosacral spinal neuron activity in response to vaginocervical-stimulation
c-Fos expression as endogenous marker of lumbosacral spinal neuron activity in response to vaginocervical-stimulation
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DOI：
10.1016/s1385-299x(01)00123-4
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发表时间：
2002-02-01
期刊：
影响因子：
--
通讯作者：
Rampin, O
中科院分区：
文献类型：
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作者：
Ghanima, A;Bennis, M;Rampin, O
本文的重点是描述一种同时刺激雌性大鼠阴道和子宫颈并记录阴道收缩的方法。还研究了发情周期对阴道宫颈敏感性的影响。使用乳胶气球，通过注射器用水充气并连接到压力传感器，使我们能够记录阴道收缩，其强度是阴道敏感性的指标，并刺激参与反射弧的脊髓神经元;在刺激结束时，将放气的气囊用作探针以进行阴道涂片，实验的时刻。Fos免疫细胞化学法鉴定活化神经元。光学显微镜计数的Fos免疫反应神经元在不同阶段的发情周期允许我们量化的阴道子宫内膜刺激的反应，并证明阴道子宫内膜敏感性的变化显着整个发情周期。这一发现证实了性激素在调节和控制阴道和子宫颈敏感性方面的重要作用，子宫颈是女性生殖道的一部分，与各种神经内分泌、行为和神经变化有关。(C)2002 Elsevier Science B. V.保留所有权利。
The focus of this paper is to describe a method for the simultaneous stimulation of the vagina and uterine cervix and recording of vaginal contractions in the female rat. The influence of the estrous cycle on vaginocervical sensitivity was also investigated. The use of a latex balloon, inflated with water via a syringe and connected to a pressure transducer allowed us to record vaginal contractions, the intensity of which is an index of vaginal sensitivity, and to stimulate those spinal neurons involved in the reflex arc; at the end of the stimulation the deflated balloon was used as a probe to perform a vaginal smear in order to determine the stage of the estrous cycle at the moment of the experiment. Activated neurons were identified by Fos-immunocytochemistry. Light microscope counting of Fos-immunoreactive neurons at different stages of the estrous cycle permitted us to quantify the response to vaginocervical stimulation and to demonstrate that vaginocervical sensitivity changes significantly throughout the estrous cycle. This finding confirms the important role of sex hormones in the modulation and control of the sensitivity of the vagina and cervix, a part of the female genital tract which is implicated in a variety of neuroendocrine, behavioral and neural changes. (C) 2002 Elsevier Science B.V. All rights reserved.